2012 Annual Report
Application of genetic analysis and TILLING (targeting induced local lesions in genomes) for identification and mapping of important traits in common bean.
Development and release of improved germplasm for specific agriculturally important biotic and abiotic stress tolerance traits and identification of associated molecular markers.
A recombinant inbred line (RIL) population (350 lines) was evaluated for response to drought in replicated trials in Nebraska and Puerto Rico. The population is currently being genotyped using SNPs (single-nucleotide polymorphism) and indel (insertion-deletions) markers. An Association Mapping (AM) population of Andean germplasm was developed as part of a Feed the Future project, increased at Tropical Agriculture Research Station (TARS), and sent to collaborators in WA, MI, MD, PR, and South Africa. Another AM population (100 lines) developed by the BeanCAP project was evaluated for a second year under drought stress. The EMS (ethyl methanesulfonate) mutant population (>2,500 lines) is being evaluated for a nutrition related seed trait at an ARS laboratory.
Two manuscripts describing the development of germplasm by the U. of Puerto Rico for virus resistance and for web blight resistance were accepted for publication in the Journal of Agriculture of the University of Puerto Rico and in the Journal of Plant Registrations, respectively. ARS-TARS participated in the evaluation of lines resulting in both releases. Advanced lines for drought, root rot, and Empoasca resistance are being tested for potential release. In addition, advanced lines of tepary (Phaseolus acutifolius) were generated, for potential release, with improved seed size and with abiotic stress tolerance.
Mcclean, P.E., Burridge, J., Beebe, S., Rao, I.M., Porch Clay, T.G. 2011. Crop improvement in the era of climate change: an integrated, multi-disciplinary approach for common bean (Phaseolus vulgaris L.). Functional Plant Biology. 38:927-933.
Beaver, J., Zapata, M., Alameda, M., Porch Clay, T.G., Rosas, J.C. 2012. Registration of PR0401-259 and PR0650-31 Dry Bean Germplasm Lines. Crop Science. 6:81-84.