Location: Tropical Crops and Germplasm Research
2011 Annual Report
Application of genetic analysis and TILLING (targeting induced local lesions in genomes) for identification and mapping of important traits in common bean.
Development and release of improved germplasm for specific agriculturally important biotic and abiotic stress tolerance traits and identification of associated molecular markers.
A recombinant inbred line (RIL) population (300 lines) was developed during the last fical year for drought evaluation and now is being used in experimental evaluations in Nebraska and Puerto Rico. An Association Mapping (AM) population was developed as part of the Bean CAP project. The RIL and the AM populations were evaluated for their response to drought in Fiscal Year 2011. Another large RIL population (300 lines) developed by ARS is being evaluated by a collaborator for work on nutrition traits. A RIL population, developed at the Tropical Agriculture Research Station (TARS) in collaboration with Michigan State for the study of the genetics of leafhopper resistance was tested in a second set of trials under leafhopper pressure in the field in Michigan and in Puerto Rico. The EMS (ethyl methanesulfonate) mutant population (>2,500 lines) is being evaluated of specific seed traits at two ARS laboratories, and at Agriculture and Agri-Food Canada.
A manuscript on the release of two black bean germplasm lines with tolerance heat and drought, and resistance to CBB and root rot, has been accepted by the Journal of Plant Registrations for publication.
Ramirez-Builes, V.H., Porch Clay, T.G., Harmsen, E.W. 2011. Evaluation of the efficiency of water use in common bean under drought and non-stress conditions in the greenhouse and field. Agronomy Journal. 103:1206-1215.
Zapata, M., Beaver, J.S., Porch Clay, T.G. 2011. Dominant gene for common bean resistance to common bacterial blight caused by Xanthomonas axonopodis pv. phaseoli. Euphytica. 179:373-382.
Pastor Corrales, M.A., Wasonga, C.J., Porch Clay, T.G., Griffiths, P.D. 2010. Targeting gene combinations for broad spectrum rust resistance in heat tolerant snap beans developed for tropical environments. Journal of the American Society for Horticultural Science. 135(6):521-532.
Porch Clay, T.G., Smith, J.R., Beaver, J.S., Griffiths, P.D., Canaday, C. 2010. Registration of TARS-HT1 and TARS-HT2 heat tolerant dry bean germplasm lines. HortScience. 45:1278-1280.
Porch Clay, T.G., Urrea, C., Beaver, J., Valentin, S., Peña, P.A., Smith, J.R. 2011. Registration of TARS-MST1 and SB-DT1 multiple-stress tolerant black bean germplasm. Journal of Plant Registrations. 6(1):75-80.