Location: Tropical Crops and Germplasm Research
2009 Annual Report
Application of genetic analysis and TILLING (targeting induced local lesions in genomes) for identification and mapping of important traits in common bean.
Development and release of improved germplasm for specific agriculturally important biotic and abiotic stress tolerance traits and identification of associated molecular markers.
Six recombinant inbred line (RIL) populations were developed for the study of the genetics of drought tolerance in common bean in collaboration with the University of Nebraska. These populations are currently in the F4 generation. The current mutant population (>2,000 lines) was sent to two ARS labs in the US for evaluation of specific seed traits, while population development continues in Puerto Rico.
The program is currently in the process of releasing a kidney bean germplasm line with heat tolerance. Generated advanced lines of BC3 and BC4 generation breeding lines with VAX 6 derived CBB resistance for potential release that are currently being tested in the US and Puerto Rico.
Porch, T.G., Ramirez, V.H., Santana, D., Harmsen, E.W. 2009. Evaluation of drought tolerance in common bean germplasm in Juana Díaz, Puerto Rico. Journal of Agronomy and Crop Science 195:328-334.
Ramirez, V.H., Porch, T.G., Harmsen, E.W. 2008. Development of linear models for estimation of leaflet area in common bean (Phaseolus vulgaris L.). J. Agric. Univ. P.R. 92(3-4):171-182.
Blair, M.W., Porch, T.G., Cichy, K.A., Galeano, C.H., Lariguet, P., Pankurst, C., Broughton, W. 2007. Induced mutants in common bean (Phaseolus vulgaris), and their potential use in nutrition quality breeding and gene discovery. Israel Journal of Plants Sciences 55:191-200.