2012 Annual Report
The second year of a general germplasm evaluation of 1357 soybean accession was planted.
We distributed 26,785 seed lots from 15,196 in response to 594 requests from 336 individuals. This is the tenth year in a row in which we have distributed more seed lots than total accessions in the Collection and are the only collection in the National Plant Germplasm System (NPGS) to have ever done this. In data combined over 11 locations in the 2010 Uniform Preliminary IIIB Test - Northern Region and 18 locations in the 2011 Uniform III Test - Northern Region, LG08-1643 yielded 97% of the best check variety, IA 3048. By pedigree, LG08-1643 is 12% wild soybean.
Seeds of 256 experimental lines derived from 63 exotic parents were sent to soybean breeders in 6 commercial companies for testing and use. Over 9 locations, LG09-8166, derived solely from exotic germplasm equaled the yield of IA4005, the highest yielding cultivar. LG10-2699 yielded 6.1 bu/a more than IA4005 over 7 locations. It has 31% of its pedigree derived from 5 introductions.
We have tentatively identified soybean lines with rust resistance transferred from G. tomentella. We are growing for the first time soybean lines with G. tomentella cytoplasm.
Improved experimental lines were developed with seed isoflavone concentrations that range from 0.62 to 6.42 mg/gm.
We conducted viral-induced gene silencing (VIGS) on three MMP2 genes that are candidate pathogen-responsive genes, as well as 8 genes identified as being candidate defense genes against the disease sudden death syndrome (SDS). We had gene expression determined for 36 different disease or pest induced soybean samples, and have analyzed 20 of them. We have reduced expression via RNA-interference (RNAi) for a soybean G-protein coupled receptor protein (GPCRP) and have plants in the T2 stage, and we made constructs to use RNAi against an MPP2 gene as well as a 14-3-3 gene, and we have sent those to our collaborator at AgCanada for generation of stable transgenics. Arabidopsis T-DNA transgenics are also being screened to determine a possible defense role of 10 genes. We have identified 11 genes out of about 38,000 that showed pathogen-specific gene expression patterns, and we have cloned three of these into Agrobacterium for overexpression in Arabidopsis.
Nelson, R.L., Johnson, E.O. 2012. Registration of high-yielding soybean germplasm line LG04-6000. Journal of Plant Registrations. 6:212-215.
Wang, D., Qi, M., Calla, B., Korban, S., Clough, S.J., Sudin, G.W., Toth, I., Cock, P.J., Zhao, Y. 2011. Genome-wide identification of genes regulated by the Rcs Phosphorelay system in Erwinia amylovora. Molecular Plant-Microbe Interactions. 25(1):6-17.
Panduranganl, S., Pajak, A., Molnar, S., Cober, E., Dhaubhadel, S., Hernandez-Sebastia, C., Kaiser, W.M., Nelson, R.L., Huber, S.C., Marsolais, F. 2012. Relationship between asparagine metabolism and protein concentration in soybean seed. Journal of Experimental Botany. 63(8):3173-3184.
Nelson, R.L., Johnson, E.O. 2011. Registration of Soybean Germplasm Line LG00-6313. Journal of Plant Registrations. 5:406-409.
Nelson, R.L., Johnson, E.O. 2011. Registration of Soybean Germplasm Line LG00-3372. Journal of Plant Registrations. 5:403-405.
Li, S., Smith, J.R., Nelson, R.L. 2011. Resistance to phomopsis seed decay identified in maturity group V soybean plant introductions. Crop Science. 51:2681-2688.
Burkey, K.O., Booker, F.L., Ainsworth, E.A., Nelson, R.L. 2012. Field assessment of a snap bean ozone bioindicator system under elevated ozone and carbon dioxide in a free air system. Environmental Pollution. 166:167-171.
Kim, K., Unfried, J.R., Hyten, D.L., Frederick, R.D., Hartman, G.L., Nelson, R.L., Song, Q., Diers, B.W. 2012. Molecular mapping of soybean rust resistance in soybean accession PI 561356 and SNP haplotype analysis of the Rpp1 region in diverse germplasm. Theoretical and Applied Genetics. 125:1339-1352.
Wang, D., Calla, B., Vimolmangkang, S., Korban, S.S., Wu, X., Huber, S.C., Clough, S.J., Zhao, Y. 2011. The orphan gene ybjN conveys pleiotropic effects on multicellular behavior and survival of Escherichia coli. PLoS One. 6(9):e25293.