1a.Objectives (from AD-416)
The proposed objectives are:. 1)to conduct on-farm surveys in organic and all natural poultry production practices to determine the prevalence and diversity of Salmonella serovars in these production systems as compared to more intensive commercial systems;. 2)to develop and evaluate intervention strategies targeting control of Salmonella during the feed withdrawal and transportation processes prior to slaughter of broiler chickens to minimize cross contamination during transport and slaughter;. 3)to evaluate post-harvest interventions to control Salmonella using novel antimicrobials in pre- and post-chiller applications, for both water and air chilling, and for finished raw products; and. 4)to develop risk assessment models that can be adapted to organic and all natural production
and processing systems. The proposed research will generate multiple publications in peer-reviewed scientific journals, presentations in meetings, and annual updates as well as a complete final report to the funding agency.
1b.Approach (from AD-416)
A minimum of 10 flocks from each production system will be sampled during grow-out. Chick box paper pads from each production system will be collected from a minimum of 10 boxes and will be sampled. Each flock will be sampled on at least 3 occasions and 6-10 of each sample type will be collected during each sampling occasion. Salmonella parameters will be evaluated on each sample collected:. 1)isolation frequency;. 2)serotype diversity;. 3)antimicrobial resistance pattern; and. 4)genotype diversity.
The on-farm surveys have been completed. A large collection of Salmonella has been isolated with serovar Kentucky being the most commonly recovered. Salmonella isolation data is currently being evaluated to determine the need for genotyping. Studies on addition of prebiotics to diets as a means to mitigate Salmonella colonization have been conducted, and results are currently being analyzed. Post-harvest interventions evaluation is ongoing, and final data is not available yet. Risk assessment models are currently being developed. The Principal Investigator monitors the progress of the study by coordinating frequent conference call meetings, in addition to individual meetings whenever team members attend scientific events throughout the year.