1a.Objectives (from AD-416):
1. Implement 454 sequencing technology to compare gene expression impacted by ecological factor affecting invasiveness.
2. To use this technology to also identify regulatory regions and potential targets for future manipulation of invasive perennial weeds.
1b.Approach (from AD-416):
Ecological factors have an impact on invasiveness of perennial weeds of the Northern plains; for example, Canada thistle and leafy spurge. These ecological factors cause changes in gene expression that are regulated by cis-acting elements. Advances in 454 sequencing technology now make it uniquely suited for the rapid and efficient quantification of both. 1)gene expression, and. 2)the identification of the cis-acting elements that control gene expression. Quantification of gene expression will be accomplished by 454 sequencing of biological samples collected from various ecological settings. This information will be analyzed and compared to obtain a dataset of genes linked to invasive traits. Further, 454 sequencing will be used to characterize genomic DNA to identify the specific cis-acting elements regulating the genes from our dataset.
The objectives of this SCA have been completed and no further research is planned. The outcomes from this SCA have been presented at numerous conferences, workshops, and meetings, and published in numerous international, peer-reviewed journals over the life of the project. In summary, this SCA provided important information on how simulated tillage impacts cross-talk among hormones to drive molecular networks regulating cell cycle processes leading to cell division, differentiation, and vegetative outgrowth. This information will be valuable for developing strategies for next-generation, integrated weed management practices.