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United States Department of Agriculture

Agricultural Research Service

Research Project: DETECTION OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHY AGENTS IN LIVESTOCK, WILDLIFE, AGRICULTURAL PRODUCTS, AND THE ENVIRONMENT Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)

Loss of prion protein leads to age-dependent behavioral abnormalities and changes in cytoskeletal protein expression - (Peer Reviewed Journal)
Schmitz, M., Greis, C., Ottis, P., Schulz-Schaeffer, W.J., Wrede, A., Onisko, B., Silva, C.J., Requena, J.R., Varges, D., Varges, D. 2014. Loss of prion protein leads to age-dependent behavioral abnormalities and changes in cytoskeletal protein expression. Electronic Publication. DOI: 10.1007/s12035-014-8655-3.
Oxidation of methionine in PrP is dependent upon the oxidant and the amino acid two positions removed(Abstract)     Reprint Icon - (Proceedings)
Silva, C.J., Dynin, I.A., Erickson-Beltran, M.L., Hui, C., Carter, J.M. 2013. Oxidation of methionine in PrP is dependent upon the oxidant and the amino acid two positions removed(Abstract). Meeting Abstract. Prion 7:81. Available: https://landesbioscience.com/journals/prion/05-Prion7-2ARB-Posters%20PS.pdf.
A comparison of the structure of the PK-sensitive and PK-resistant forms of PrPSc(Abstract) - (Abstract Only)
Silva, C.J., Sajnani, G., Ramos, A., Pastrana, M.A., Onisko, B.C., Erickson-Beltran, M.L., Antaki, E.M., Dynin, I.A., Vazquez-Fernandez, E., Sigurdson, C.J., Carter, J.M., Requena, J.R. 2013. A comparison of the structure of the PK-sensitive and PK-resistant forms of PrPSc(Abstract). Meeting Abstract. PS 26.
Distinguishing between PrPC and PrPSc using small molecule reagents(Abstract) - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Dynin, I.A., Carter, J.M. 2013. Distinguishing between PrPC and PrPSc using small molecule reagents(Abstract). Meeting Abstract. PS 25.
Oxidation of methionine 216 in sheep and elk prion protein is highly dependent upon the amino acid at position 218 but is not important for prion propagation     Reprint Icon - (Peer Reviewed Journal)
Silva, C.J., Dynin, I.A., Erickson-Beltran, M.L., Requena, J.R., Balachandran, A., Onisko, B.C., Hui, C., Carter, J.M. 2013. Using mass spectrometry to detect prions and oxidized prions in scrapie-infected sheep and CWD-infected elk. Biochemistry. 52:2139-2147. doi:10.1021/bi3016795.
Structural organization of mammalian prions as probed by limited proteolysis - (Peer Reviewed Journal)
Vasquez-Fernandez, E., Alonso, J., Pastrana, M.A., Ramos, A., Stitz, L., Vidal, E., Dynin, I.A., Petsch, B., Silva, C.J., Requena, J.R. 2012. Structural organization of mammalian prions as probed by limited proteolysis. PLoS One. 7(11):e50111. doi:10.1371/journal.pone.0050111.
Rapid multiplex immunoassay to distinguish botulinum neurotoxin serotypes on a single lateral flow device(Abstract) - (Abstract Only)
(04-Aug-12)
Novel epitopes identified by Anti-PrP monoclonal antibodies produced following immunization of Prnp0/0 Balb/cJ mice with purified scrapie prions - (Peer Reviewed Journal)
Stanker, L.H., Scotcher, M.C., Lin, A.V., Mcgarvey, J.A., Prusiner, S., Hnasko, R.M. 2012. Novel epitopes identified by Anti-PrP monoclonal antibodies produced following immunization of Prnp0/0 Balb/cJ mice with purified scrapie prions. Hybridoma. 31(5):312-324. doi:10.1089/hyb.2012.0022.
Detecting and discriminating among pathogenic protein conformers(prions), using mass spectrometry-based and antibody-based approaches(Abstract) - (Abstract Only)
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Hui, C., Carter, J.M. 2012. Detecting and discriminating among pathogenic protein conformers(prions), using mass spectrometry-based and antibody-based approaches(Abstract). Meeting Abstract. Poster 216.
Probing the structure of GPI-less PrPSc by limited proteolysis(Abstract) - (Abstract Only)
Vazquez-Fernandez, E., Pastrana, M., Ramos, A., Alonso, J., Stitz, L., Vindal, E., Dynin, I.A., Silva, C.J. 2012. Probing the structure of GPI-less PrPSc by limited proteolysis(Abstract). Meeting Abstract. Prion 6(supplement),26-27.
Using synthetic small molecule reagents and antibodies to distinguish among PrP conformers: new uses for old antibodies - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Dynin, I.A., Carter, J.M. 2012. Using synthetic small molecule reagents and antibodies to distinguish among PrP conformers: new uses for old antibodies. Meeting Abstract. Prion 6(supplement),90.
Molecular approaches to detecting and discriminating among prions, a class of pathogenic molecules(Abstract) - (Abstract Only)
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Hui, C., Carter, J.M. 2012. Molecular approaches to detecting and discriminating among prions, a class of pathogenic molecules(Abstract). Meeting Abstract. Poster, 615.
Rapid and selective detection of botulinum neurotoxin serotypes-A and –B with a single immunochromatographic test strip - (Peer Reviewed Journal)
Ching, K.H., Lin, A.V., Mcgarvey, J.A., Stanker, L.H., Hnasko, R.M. 2012. Rapid and selective detection of botulinum neurotoxin serotypes-A and –B with a single immunochromatographic test strip. Journal of Immunological Methods. 380:23-29.
Human and rat brain lipofuscin proteome - (Peer Reviewed Journal)
Otts, P., Koppe, K., Onisko, B.C., Dynin, I.A., Arzberger, T., Kretzschmar, H., Requena, J.R., Silva, C.J., Huston, J.P., Korth, C. 2012. Human and rat brain lipofuscin proteome. Proteomics. 12(15-16):2445-2454. doi:10.1002/pmic.201100668.
PK-sensitive PrPSc is infectious and shares basic structural features with PK-resistant PrPSc     Reprint Icon - (Peer Reviewed Journal)
Sajnani, G., Silva, C.J., Ramos, A., Pastrana, M.A., Onisko, B.C., Erickson-Beltran, M.L., Antaki, E.M., Sigurdson, C.J., Carter, J.M., Requena, J.R. 2012. PK-sensitive PrPSc is infectious and shares basic structural features with PK-resistant PrPSc. PLoS Pathogens. 8(3):e1002547. doi:10.1371/journal.ppat.1002547.
Using small molecule reagents to selectively modify epitopes based on their conformation - (Peer Reviewed Journal)
Silva, C.J. 2012. Using small molecule reagents to selectively modify epitopes based on their conformation. Prion. 6:(2)165-175.
A rapid method to improve protein detection by indirect ELISA - (Peer Reviewed Journal)
Hnasko, R.M., Lin, A.V., Mcgarvey, J.A., Stanker, L.H. 2011. A rapid method to improve protein detection by indirect ELISA. Biochemical and Biophysical Research Communications. 410(4):726-731. doi: 10.1016/j.bbrc.2011.06.005.
Using Mass Spectrometry to Diagnose Prion diseases: Can we do that? - (Abstract Only)
(16-May-11)
Diagnosing Prion Diseases: Mass Spectrometry-Based Approaches - (Abstract Only)
Silva, C.J., Erickson, M.L., Dynin, I.A., Onisko, B.C., Carter, J.M. 2011. Diagnosing Prion Diseases: Mass Spectrometry-Based Approaches. Meeting Abstract. Prion, Volume 5 (Supplement), April/May/June 2011. page 85.
Probing structural differences between PrPC and PrPSc by surface nitration and acetylation: evidence of conformational change in the C-terminus - (Peer Reviewed Journal)
Gong, B., Ramos, A., Vazquez-Fernandez, E., Silva, C.J., Alonso, J., Requena, J.R. 2011. Probing structural differences between PrPC and PrPSc by surface nitration and acetylation: evidence of conformational change in the C-terminus. Biochemistry. 50:4963-4972.
Mass spectrometry and prions: The need to simplify and remove oil from the system - (Review Article)
Silva, C.J. 2010. Mass spectrometry and prions: The need to simplify and remove oil from the system. International News on Fats, Oils and Related Materials.21(8):517-520.
Detecting and quantifying prions: Mass spectrometry-based approaches - (Abstract Only)
(20-Apr-10)
Assessing the Role of Oxidized Methionine at Position 213 in the Formation of Prions in Hamsters - (Peer Reviewed Journal)
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L., Vensel, W.H., Requena, J., Antaki, E.M., Carter, J.M. 2010. Assessing the Role of Oxidized Methionine at Position 213 in the Formation of Prions in Hamsters. Journal of Biochemistry. (49):1854-1861.
Mass Spectrometry of Prions: Approaches to Conformational Distinction - (Abstract Only)
Silva, C.J., Onisko, B., Dynin, I.A., Erickson, M.L., Carter, J.M. 2009. Mass Spectrometry of Prions: Approaches to Conformational Distinction. [Abstract]. AOAC 123rd Annual Meeting & Exposition. S-902. p68-69
Disinfectants and Prions - (Abstract Only)
Silva, C.J. 2009. Disinfectants and Prions. [Abstract]. AOAC Annual Meeting & Exposition S-401. p63
Prion Infectivity Assays. - (Government Publication)
(30-Mar-09)
Mass Spectrometric Approaches to Detecting and Quantifying Prions - (Abstract Only)
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L. 2009. Mass Spectrometric Approaches to Detecting and Quantifying Prions. [Abstract]. PrP Canada p28.
Prion diseases: A little prevention can prevent a catastrophe - (Abstract Only)
Silva, C.J. 2009. Prion diseases: A little prevention can prevent a catastrophe. [Abstract]. AOAC AM-1 p1.
Inoculation of Scrapie with the Self-Assembling RADA-Peptide Disrupts Prion Accumulation and Extends Hamster Survival - (Peer Reviewed Journal)
Hnasko, R.M., Bruederle, C.E. 2009. Inoculation of Scrapie with the Self-Assembling RADA-Peptide Disrupts Prion Accumulation and Extends Hamster Survival. PLoS ONE. 4(2): http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004440
Effects of anaerobic digestion and aerobic treatment on gaseous emissions from dairy manure storages - (Peer Reviewed Journal)
Zhang, R., Mcgarvey, J.A., Mitloehner, F. 2008. Effects of anaerobic digestion and aerobic treatment on gaseous emissions from dairy manure storages. International Journal of Agricultural & Biological Engineering. 1(2):15-20.
Prions: The Chemistry of Infectious Proteins - (Abstract Only)
Silva, C.J. 2008. Prions: The Chemistry of Infectious Proteins [Abstract]. American Oil Chemists' Society. S-401. p.63.
Mass spectrometric approaches to detecting prions and protein conformers - (Abstract Only)
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L. 2009. Mass spectrometric approaches to detecting prions and protein conformers. [Abstract] AOAC S-401. p63
Prion Diseases - (Book / Chapter)
Silva, C.J. 2009.Prion Diseases:Sequelae and Long Term Consequences of Infectious Diseases. District of Columbia:ASM Press. 442p.
The Nuclear Transcription Factor RAR Associates with Neuronal RNA Granules and Suppresses Translation - (Peer Reviewed Journal)
Onisko, B.C., Chen, N., Napoli, J. 2008. The Nuclear Transcription Factor RAR Associates with Neuronal RNA Granules and Suppresses Translation. Journal of Biological Chemistry. 283(30):20841-20847.
Mass Spectrometric Approaches to Detecting and Quantifying Prions - (Abstract Only)
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L., Carter, J.M. 2008. Mass Spectrometric Approaches to Detecting and Quantifying Prions. [Abstract]. Third International CWD Symposium. Advancing the Science and Developing the Tools. Vol 3. p23.
Last Modified: 7/28/2014
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