2010 Annual Report
1)identify new genes for FHB resistance in Asian landraces and U.S. hard winter wheat germplasm lines, and.
2)transfer and pyramid genes for FHB resistance from diverse sources into locally adapted cultivars using marker-assisted selection. Two recombinant inbred line (RIL) populations from the crosses between two Chinese landraces, Haiyanzhong and Huangfangzhu, and Wheaton (a highly susceptible parent) were evaluated for FHB resistance. The data were analyzed and quantitative trait locus (QTL) analysis showed that Haiyanzhong has a QTL on wheat chromosome 7D and explained about 22-30% of variation for percentage of scabbed spikes (PSS). In Huangfangzhu, two QTLs with major effects on PSS were mapped on chromosome 3BS (near resistance gene Fhb1) and on chromosome 7AL. The manuscripts for the mapping work are in preparation.
A RIL mapping population (cultivar Heyne x Trego) was evaluated for FHB in both field and greenhouse for FHB resistance. Heyne is a local HWW cultivar with moderate resistance to FHB. Parents were screened with 1000 DNA markers to identify polymorphisms. Work will continue identifying chromosome regions that affect disease severity.
A new QTL was mapped on chromosome 7A near the centromere using a population of 200 chromosome recombinant inbred lines. This QTL showed a larger effect on PSS and explained 25% of the phenotypic variation. Progress on this agreement is monitored by regularly discussing program goals, approaches, and results (on site) and by reviewing annual accomplishments reports.