2009 Annual Report
1a.Objectives (from AD-416)
Develop stress, disease, and pest-tolerant cultivars of common and underutilized landscape trees suitable for urban areas and height-restricted planting sites.
Develop disease- and insect-screening assays for identifying resistant parent taxa and hybrid progeny.
Identify interspecific and intergeneric barriers to introgression of desired traits into adapted germplasm.
Develop non-invasive tree cultivars, via wide-hybridization and inter-ploid crosses, to limit naturalization and gene-introgression into natural populations.
Quantify genome sizes and ploidy levels in related taxa and identify parental taxa for interploid crosses.
Develop methods for ploidy-manipulation of vegetative meristems to facilitate interploid crosses and ploidy bridges.
Use molecular techniques for hybrid verification and genetic-relatedness tests within cultivated germplasm of important tree species.
1b.Approach (from AD-416)
Develop in vitro and in situ disease and insect screening assays for identifying resistant parent taxa and hybrid progeny; determine inter- and intrageneric barriers to introgression of resistance and ornamental traits into adapted germplasm via controlled pollinations and fluorescence microscopy; quantify genome sizes and ploidy levels using flow cytometry and manipulate ploidy level via mitotic inhibitors; verify hybrid and cultivar parentage using molecular markers. Evaluate progenies and make clonal selections for pest resistance, stress tolerances, non-invasiveness, and ornamental traits. Test plants for geographic and climatic adaptability and horticultural traits through cooperative procedures.
A long-term breeding program for Catalpa has begun to develop disease-tolerant, non-invasive cultivars with novel ornamental traits. Controlled pollinations were conducted on novel hybrid combinations, including intraspecific, interspecific, and intergeneric crosses. Progeny from prior year’s Catalpa crosses, including first triploid populations of Chitalpa, were germinated and established. Genetic diversity and molecular identification of Catalpa germplasm using AFLP markers has begun. Nyssa breeding has continued, utilizing an isolation block of elite F1s for generation of a large quantity of F2 seed segregating for improved plant habit, fall color, and leaf spot tolerance. Molecular identification of a leaf spot organism from Nyssa is underway. A two-year study testing resistance of container-grown Tsuga hybrids to hemlock woolly adelgid has been initiated. Test crosses in Tsuga hybrids have been made for developing advanced generation hybrids. Flow cytometric analysis continues on Catalpa, Celtis, and Ulmus americana. Molecular markers developed previously are being used to analyze genetic diversity among cultivated and non-cultivated Chionanthus.
Montgomery, M.E., Bentz, S.E., Olsen, R.T. 2009. Evaluation of hemlock (Tsuga) species and hybrids for resistance to Adelges tsugae (Hemiptera: Adelgidae) using artificial inoculation. Journal of Economic Entomology. 102:1247-1254.