2010 Annual Report
The molecular basis of Hessian fly virulence/avirulence will be approached by determining the functions of secreted salivary gland proteins (SSGP) of Hessian fly in virulence or avirulence to wheat. Differential gene expression of SSGPs will be tested in different biotypes of Hessian fly using a custom-designed microarray. Candidate genes for virulence or avirulence effectors will be identified. Candidate genes will be tested by gene silencing using RNA interference.
Progress continued on the effort to clone the wheat resistance gene Hdic, which confers resistance to Hessian fly. Candidate genes have been identified and are being tested.
We discovered a rapid mobilization of membrane lipids in resistant plants in response to Hessian fly attack. Mobilized lipids are likely converted into defense signaling molecules and components for cell wax. This discovery suggests that lipid mobilization and conversion might be a necessary molecular basis for plants to be resistant to Hessian fly. This work is among the first studies for systematic analysis of membrane lipids in plant defense.
Progress on this agreement is monitored by regularly discussing program goals, approaches, and results (teleconference, email) and by reviewing annual accomplishments reports.