Location: Grape Genetics Research
2008 Annual Report
Functional genomics technology development Evaluating grapevine gene function is challenging due to the lengthy transformation process. In order to accelerate functional gene testing, we adapted a transient gene expression system (previously used in one grape species) for use in multiple species, including those with powdery mildew resistance. Confirmed gene function will be a platform for identifying and utilizing genes for foliar disease resistance, permitting selection of improved varieties.
Whole plant functional testing of grapevine genes is challenging due to large plant size and the time required from transformation to fruiting. In order to develop a small, easily transformed grapevine variety, we evaluated the impact of ten allelic forms of the grape GAI gene on plant growth and development in the model plant Arabidopsis. We observed that different grape GAI gene allelic forms have different impacts on Arabidopsis growth, architecture and flowering time. This observation advanced our understanding of grape GAI gene function and provides an opportunity to create a new variety suited for grape functional genomics research. NP 301 Plant Genetic Resources, Genomics and Genetic Improvement Component 2: Crop Informatics, Genomics, and Genetic Analyses Problem Statement 2B: Structural Comparison and Analysis of Crop Genomes. Problem Statement 2C: Genetic Analyses and Mapping of Important Traits.
Grape Scion Disease Resistance Characterization Developing grapes can be attacked by pathogens that reduce grape quality and yield. The pathogens interact with the cuticle, the outer layer of the grape. In order to identify possible disease resistance components in the cuticle, we characterized cuticle development and resolved and identified proteins from wild and cultivated grapes at several developmental stages. The new information on cuticle biochemistry and development will enable isolation of specific components that condition pathogen resistance and impact berry growth. NP 301 Plant Genetic Resources, Genomics and Genetic Improvement Component 2: Crop Informatics, Genomics, and Genetic Analyses Problem Statement 2C: Genetic Analyses and Mapping of Important Traits. NP 303 Plant Diseases Component 3: Plant Disease Resistance Problem Statement 3A. Mechanisms of Plant Disease Resistance.
Gee, C., Gadoury, D., Cadle Davidson, L.E. 2008. Ontogenic resistance to Uncinula necator varies by genotype and tissue type in a diverse collection of Vitis spp. Plant Disease. 92:1067-1073.