1a.Objectives (from AD-416):
To evaluate the adjuvant effects of Seppic's immunomodulators in poultry immune system.
1b.Approach (from AD-416):
Broiler chickens will be treated with Seppic's immunomodulators and the effects on avian immune response to mucosal pathogens will be evaluated using established disease parameters and various immunological assays.
This 5-year trust agreement led to several new findings concerning the mechanisms of adjuvant action. Using Eimeria profilin recombinant antigen as a model antigen, we evaluated 6 Montanide(TM) adjuvants (ISA 70, ISA 71, ISA 201, ISA 206, Montanide Gel, IMS) in broilers. First, we found that ISA71 elicited most potent T-cell response which was protective against live E. acervulina infection. Second, profilin plus ISA 71 reduced fecal oocyst shedding compared with vaccination in the absence of adjuvant. Third, all adjuvants enhanced profilin serum antibody titers and increased levels of gene transcripts encoding IL-2, IL-10, IL-17A, and IFN-y. Fourth, recombinant profilin subunit vaccine, when given in combination with ISA 71 VG, augmented protective immunity against both E. acervulina and E. tenella, and finally aqueous nanoparticle-based IMS 1313 N VG (IMS 1313) adjuvant in combination with a profilin subunit vaccine augmented protective immunity against multiple Eimeria infections. The results of these studies clearly indicated that adjuvant-profilin complex is superior to profilin alone in eliciting protective cell-mediated immunity to coccidiosis.