Location: Horticultural Crops Research
2012 Annual Report
Our ongoing germplasm project with Rubus occidentalis that began with the collection of germplasm in the Midwestern and southern United States has led to the identification of sources of aphid resistance that should impart resistance to Black raspberry necrosis virus. Anthocyanins and phenolics were determined for this germplasm. In an SCRI blueberry project coordinated by the USDA-ARS-PSI, we phenotyped a population that will be genotyped for developing marker assisted breeding in blueberry. In our SCRI funded RosBreed project, we are working on the potential for marker assisted breeding in strawberry by merging phenotyping and genotyping information. We identified Reference Sets that includes 800+ genotypes, coordinated their propagation and distribution. This past summer, we conducted the first year of phenotyping on this germplasm at our location. We will be compiling the data from all locations (FL, CA, MI, and NH) this fall.
A virus associated with Blueberry necrotic blotch disease in southern highbush blueberry in Georgia was characterized. The virus has two helicase genes, which is unique among all known viruses. Multiple sets of primers for detection of the virus were developed and evaluated each set against 55 isolates of the virus from NC, GA, FL, AR and MS. Two sets of primers detected all 55 isolates and are being used for detection of the virus in research and certification programs. Transmission experiments are being done by collaborators in FL and GA since the disease is present in those areas.
We continued field trials of raspberry with mixed virus infections to determine the role of various viruses on plant growth, fruit yield and quality and to examine synergistic interactions between three viruses that commonly infect red raspberry in the Pacific Northwest. We are in the third year of the study and mid-way through harvests; the results from this year appear to be consistent with those from 2011. Raspberry bushy dwarf virus (RBDV) in combination with Raspberry leaf mottle virus (RLMV) has the greatest impact on plant growth, but the combination has fruit quality and yield comparable to single infections with RBDV. In contrast, RBDV plus Raspberry latent virus (RpLV) has a much great impact on fruit yield and quality compared to RBDV alone. There does not appear to be an interaction between RLMV and RpLV viruses in terms of plant growth or fruit quality. Also in mixed infections it was found that RLMV resulted in approximately a 400-fold increase in the concentration of RBDV. Concentrations of the other viruses were not altered in mixed infections.
Finn, C.E., Kempler, C., Moore, P., Strik, B.C., Yorgey, B.M., Martin, R.R., Galletta, G.J. 2011. 'Sweet Bliss’ strawberry. HortScience. 46(12):1701-1705.
Moore, P.P., Hoashi-Erhardt, W., Finn, C.E., Kempler, C. 2011. ‘Puget Crimson’ Strawberry. HortScience. 46(12):1698-1700.
Kempler, C., Hall, H., Finn, C.E. 2012. Raspberry. In: Badenes, M.L. and Byrne, D.H., editors. Handbook of Plant Breeding: Vollume 8: Fruit breeding. Springer, New York. p. 151-190.
Clark, J.R., Finn, C.E. 2011. Blackberry breeding and genetics. In: Flachowsky, H. and Hanke, V.M., editors. Methods in Temperate Fruit Breeding. Fruit, Vegetable, and Cereal Science and Biotechnology 5 (Special Issue 1). Global Science Books, Ltd. UK. p. 27-43.
Finn, C.E., Clark, J.R. 2012. Blackberry. In: Badenes, M.L. and Byrne, D.H., editors. Handbook of Plant Breeding: Volume 8: Fruit breeding. Springer, New York. p. 151-190.
Finn, C.E., Clark, J.R. 2011. Emergence of blackberry as a world crop. Chronica Horticulturae. 51:13-18.
Dossett, M., Bassil, N.V., Lewers, K.S., Finn, C.E. 2012. Genetic diversity in wild and cultivated black raspberry evaluated by simple sequence repeat markers. Genetic Resources and Crop Evolution. DOI:10.1007/s10722-012-9809-8.
Quito-Avila, D., Martin, R.R. 2012. Real-time RT-PCR for detection of Raspberry bushy dwarf virus, Raspberry leaf mottle virus and characterizing synergistic interactions in mixed infections. Journal of Virological Methods. 179(1):38-44.
Quito-Avila, D.F., Lightle, D., Lee, J.C., Martin, R.R. 2012. Transmission biology of Raspberry latent virus, a plant reovirus vectored by the aphid Amphorophora agathonica. Phytopathology. 102:547-553.
Strik, B.C., Clark, J.R., Finn, C.E., Buller, G. 2012. Management of primocane-fruiting blackberry – impacts on yield, fruiting season, and cane architecture. HortScience. 47-593-598.
Rowland, L.J., Bell, D.J., Alkharouf, N., Bassil, N.V., Drummond, F., Beers, L., Buck, E., Finn, C.E., Graham, J., Mccallum, S., Hancock, J., Olmstead, J., Main, D. 2012. Generating genomic tools for blueberry improvement. International Journal of Fruit Science. 12:276-287.
Martellie, G.P., Agranovsky, A.A., Bar-Joseph, M., Boscia, D., Candresse, T., Coutts, R., Dolja, V.V., Hu, J., Jelkmann, W., Karasev, A.V., Martin, R.R., Minafra, A., Namba, S., Vetton, H.J. 2012. Closteroviridae. In: King, A.M.Q., Adams, M.J., Carstens, E.B. and Lefkowitz, E.J. editiors. Virus Taxonomy: Classification and Nomenclature of Viruses. Ninth Report of the International Committee on Taxonomy of Viruses. San Diego. Elsevier Academic Press. p.987-1011.