1a.Objectives (from AD-416)
The objective of this cooperative research project is to advance cotton genomics by developing the resources for the genome sequence of the Upland cotton (Gossypium hirsutum) genetic standard, TM-1.
1b.Approach (from AD-416)
Large DNA-insert BAC libraries of the highly inbred genetic standard, TM-1, will be constructed and assembled into contigs to create a physical map. Minimum tiling path (MTP) BAC clones from selected contigs will be sequenced, and the assembled and annotated data will provide the complete cotton genome sequence with the gene content and distribution.
The goal of this project is to develop resources for genome sequencing of the Gossypium hirsutum Upland cotton standard, TM-1, and to sequence this genetic standard for enhanced cotton improvement. In FY 2011, project scientists and collaborators made significant progress in final analysis of about 28 million base pairs of assembled nuclear DNA from chromosomes 12 and 26 of the complex Upland cotton genome. Detailed annotation of the sequence data was conducted that revealed the genome complexity and identified more than 1,000 genes of interest. Work by the project in FY 2011 established the similarity or co-linearity between the sequences of two subgenomes present in cotton and made comparisons with other model plant genomes that revealed a high-level of transposable elements that cause increased recombination in Upland cotton. Work by this project, as it continues, will provide foundational data and direction to ongoing efforts to sequence the entire cotton genome. The work will lead to an eventual highest resolution possible map of the tetraploid cotton genome, which is much needed by the worldwide cotton research community in support of their work to improve the agronomic and quality traits of this important fiber, food, and feed crop.
The ADODR monitors the cooperator's performance by e-mail communications, telephone conversations, and through critical review and analysis of shared data. More intensive interactions with the cooperator occur, at least once a year, via face-to-face interactions that include worksite reviews and appropriate discussions of research progress, problems, and priorities.