2011 Annual Report
Sub-objective 1b. Waxy lines have been provided to collaborators at Kansas State, UC Berkeley, and the Agricultural Research Institute for South-East Region of Russia. A manuscript was published on the contributions of protein and starch chemistry on digestibility, and another submitted describing the effects of waxy sorghum on ethanol production. Field trials have been completed on a set of waxy/wild-type isolines, R-lines, A/B-lines, and another set of isolines with wxa and wxb alleles.
Sub-objective 1c. BC1S2 families were planted to the field in spring 2011. Selections will be made at the end of the current growing season.
Sub objective 2a. Assessment of bmr-6 gene expression was completed, and analysis of genomic data is ongoing.
Sub objective 2b. A sorghum nuclear extract protocol was not developed because promoter elements were not conserved within lignin biosynthetic genes. An alternative strategy was developed using a putative lignin regulatory factor SbMYB68, selected based on comparative genomics. This factor is under investigation.
Sub-objective 3a. Procedures for molecular analyses of Fusarium, Alternaria, and Curvularia spp. were established. A manuscript is in preparation for characterization of Alternaria, and Curvularia spp. A procedure for collection of fungi from air was established, and a manuscript for Fusarium spp. published. One-hundred forty four sequences were submitted to GenBank.
Sub-objective 3b. Near isogenic plant color line analyses were completed and the resulting manuscript submitted. Screening grain from waxy accessions for fungal colonization, and fungal identifications, approx one-third completed. “Clean” grain for in vitro grain infections assays were produced in the greenhouse.
Sub-objective 3c. Stalk rot analyses of bmr near isolines (bmr6, bmr12, bmr6 brm12 double mutants) in two genetic backgrounds using pathogens, Fusarium thapsinum and F. verticillioides, were completed. A similar study using F. proliferatum is underway. Assays to establish conditions for inoculation by Macrophomina phaseolina have been completed and stalk rot analyses with the near isolines are underway.
Sub-objective 3d. RFLP analysis of 24 antibiotic producing fluorescent Pseudomonas spp. nearly complete. Inhibition assays with these isolates against five sorghum fungal pathogens completed.
Sahoo, L., Schmidt, J.J., Pedersen, J.F., Lee, D.L., Lindquist, J.L. 2010. Growth and fitness components of wild X cultivated Sorghum bicolor (Poaceae) hybrids in Nebraska. American Journal of Botany. 97(10): 1610-1617.
Wong, J.H., Marx, D.B., Wilson, J.D., Lemaux, P.G., Buchanan, B.B., Pedersen, J.F. 2010. Principal component analysis and biochemical characterization of protein and starch reveal primary targets for improving sorghum grain. Plant Science. Volume 179 (2010) 598-611.
Funnell-Harris, D.L., Pedersen, J.F. 2011. Presence of Fusarium spp. in air and soil associated with sorghum fields. Plant Disease 95: 648-656.
Goff, B., Moore, K., Fales, S., Nikolau, B., Pedersen, J.F. 2011. Comparison of the Use of Gas Chromotography, Spectrophotometry, and Near Infrared Spectropscopy to Quantify Prussic Acid Potential in Forages. Journal of Agriculture and Food Chemistry. 1523-1526.