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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC IMPROVEMENT OF MAIZE AND PEARL MILLET FOR RESISTANCE TO INSECTS AND AFLATOXIN

Location: Crop Genetics and Breeding Research

2011 Annual Report


1a.Objectives (from AD-416)
The long-term objective of this project is to develop corn and pearl millet germplasm with reduced aflatoxin contamination and insect damage in order to increase the productivity and quality of grains produced in the southeastern United States. Over the next five years we will:.
1)Identify new sources of germplasm with resistance to Aspergillus flavus/aflatoxin contamination, and to multiple ear-feeding insect damage;.
2)Determine biochemical and physiological bases and associated key phenotypic traits conferring resistance/ susceptibility to chewing and piercing-sucking insects in corn and pearl millet germplasm;.
3)Identify quantitative trait loci associated with reduced aflatoxin accumulation in corn; and.
4) Develop maize germplasm with desirable agronomic traits and reduced aflatoxin accumulation and increased resistance to ear-feeding insects for use in the Coastal Plain region.


1b.Approach (from AD-416)
In comparison with molecular (gene to trait, or top-down) approach, a trait to gene (bottom-up) approach will be taken for this project. Diverse (exotic and temperate) germplasm will be screened under field conditions for resistance to aflatoxin and insect pests (i.e., corn earworm, fall armyworm, maize weevil, and stink bugs). Field-proven germplasm entries will be further evaluated for key unique phenotypic traits in relation to resistance mechanisms and underlying genetics using both conventional and molecular techniques. Following rigorous field and laboratory screenings, selected germplasm will be further examined for biochemical and physiological bases that confer resistance to multiple insect pests and aflatoxin. Insect resistance mechanisms in the selected corn and pearl millet germplasm will be categorized and the biochemical and physiological processes that confer certain insect resistance will be identified. Segregating populations with resistance to aflatoxin contamination and multiple insect damage will be developed. These new populations will serve as the sources of favorable alleles for ultimately developing aflatoxin- and insect-resistant corn inbred lines with elite agronomic traits, which will improve corn production under biotic and abiotic stresses in our region.


3.Progress Report
Activities focused on the genetic improvement of corn and pearl millet for yield-related traits, resistance to A. flavus and aflatoxins, and insect pests. Brazilian maize introductions were evaluated for resistance to lepidopteran pests. Temperate corn inbreds were evaluated for corn rootworm and fall armyworm resistance. New elite Genetic Enhancement of Maize (GEM) inbreds and hybrids selected from Iowa, North Carolina, and Texas programs were evaluated for resistance to whorl and ear feeding insects. The previously evaluated GEM inbreds and hybrids from Texas and Iowa are evaluated for the foliage feeding insects in 2010. Fall armyworm and southern rust-resistant inbreds from the FAWCC(C5) population were advanced. High maysin experimental hybrids were evaluated for resistance to feeding by ear insects (corn earworm, stinkbug, maize weevil) and fall armyworm, as well as for natural enemy attraction in whorl stage. Introduced Chinese inbreds were evaluated for resistance to ear feeding insects, and rust and smut. The co-occurrence of aflatoxin contamination and maize weevil and stinkbug distributions in fields of AgraTech 760RR was assessed. Inoculation methods to allow for more effective screening for Aspergillus flavus and aflatoxin contamination were assessed. Approximately 2500 grain samples were analyzed for aflatoxin using the Vicam procedure. Approximately 500 hybrids were evaluated in 3 environments with the goal of combining aflatoxin, insect, and drought resistance in improved genetic backgrounds. Forty selections were intermated to initiate 1200 phase 2 breeding populations evaluated for agronomic traits, drought tolerance, and tolerance to late-season pests and diseases.


Review Publications
Ni, X., Wilson, J.P., Buntin, G., Guo, B., Krakowsky, M.D., Lee, R., Cottrell, T.E., Scully, B.T., Huffaker, A., Schmelz, E.A. 2011. Spatial patterns of aflatoxin levels in relation to ear-feeding insect damage in pre-harvest corn. Toxins. 3(7):920-931.

Ni, X., Sparks, A.N., Riley, D.G., Li, X. 2011. Impact of edible oils on ear pests of sweet corn. Journal of Economic Entomology. 104:956-964.

Schmelz, E.A., Kaplan, F., Huffaker, A., Dafoe, N.J., Vaughan, M.M., Ni, X., Rocca, J.R., Alborn, H.T., Teal, P.E. 2011. Identity, regulation, and activity of inducible diterpenoid phytoalexins in maize. Proceedings of the National Academy of Sciences. 108(13):5455-5460.

Du, E., Ni, X., Zhao, H., Li, X. 2010. Natural history and intragenomic dynamics of the Transib transposon Hztransib in the cotton bollworm Helicoverpa zea. Insect Molecular Biology. 20:291-301.

Guo, B.Z., Krakowsky, M.D., Ni, X., Scully, B.T., Lee, R.D., Coy, A.E., Widstrom, N.W. 2011. Registration of maize inbred line GT603. Journal of Plant Registrations. 5(2):211-214.

Ni, X., Da, K., Buntin, G., Cottrell, T.E., Tillman, P.G., Olson, D.M., Powell, R., Lee, R.D., Wilson, J.P., Scully, B.T. 2010. Impact of brown stink bug (Heteroptera: pentatomidae) feeding on corn grain yield components and quality. Journal of Economic Entomology. 103:2072-2079.

Williams, W.P., Windham, G.L., Krakowsky, M.D., Scully, B.T., Ni, X. 2010. Aflatoxin accumulation in BT and non-BT maize testcrosses. Journal of Crop Improvement. 24:392-399.

Cottrell, T.E., Wood, B.W., Ni, X. 2010. Application of plant growth regulators mitigates chlorotic foliar injury by the black pecan aphid (Hemiptera: Aphididae). Pest Management Science. 66:1236-1242.

Ni, X., Li, X., Chen, Y., Guo, F., Feng, J., Zhao, H. 2010. Metamorphosis of cisgenic insect resistance research in the transgenic crop era. In: Liu, T.-X. and Kang, L., editors. Recent Advances in Entomological Research: From Biology to Pest Management. Beijing, China:Higher Education Press. p. 157-169.

Li, X., Ni, X. 2010. Deciphering the plant-insect phenotypic arms race. In: Liu, T.-X. and Kang, L., editors. Recent Advances in Entomological Research: From Molecular Biology to Pest Management. Beijing, China:Higher Education Press. p. 3-20.

Chen, Y., Ni, X. 2010. Nitrogen modulation on plant direct and indirect defenses. In: Liu, T.-X. and Kang, L., editors. Recent Advances in Entomological Research: From Molecular Biology to Pest Management. Beijing, China:Higher Education Press. p. 52-60.

Zhang, C., Luo, X., Ni, X., Zhang, Y., Li, X. 2010. Functional characterization of cis-acting elements mediating flavone-inducible expression of CYP321A1. Insect Biochemistry and Molecular Biology. 40:898-908.

Last Modified: 9/2/2014
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