2011 Annual Report
1a.Objectives (from AD-416)
Improvement of soybean seed composition through incorporation of new genes from exotic germplasm and wide crosses. These funds will directly support the soybean project objective #4. The Milestone: release germplasm by 2011.
1b.Approach (from AD-416)
North elite (HC99-2846 and HC01-289 and HF02-005 - high-yielding Ohio lines) by south high-protein (GSF-114 from Roger Boerma, N01-10974 from Tommy Carter, Danbaekong from South Korea) crosses will be made in 2007. HC-01-289 is a MG-III high-protein (44%) Ohio line resealed in 2006. The F1 and F2 generations will be grown in Puerto Rico to give single seed decent F3 seeds. We will grow F3 plants in Wooster in 2008, classify plants for maturity and harvest individual plants. Seed from each plant will be analyzed for protein and oil content, and lines with >46% protein will be selected for further testing. Seeds of selected lines will be increased in Puerto Rico. In 2009, we will test two row plots in a replicated trial and select lines with desirable agronomic traits and high-protein content. In 2010 and 2011, we will conduct multi-row replicated trial of selected lines with check cultivars in at least two locations. Seed yield, protein, and other agronomic traits will be measured, seeds will be increased, and the best lines will be released as germplasm. More crosses will be made as new sources of high-protein become available from collaborators (e.g., soja derived high-protein line from Istvan Rajcan Canada).
In 2010, twenty advanced breeding lines (F4:8) from the cross of GSF-114 to Ohio food grade cultivars yielded more than MG-II and MG-III check cultivars in a replicated trial. These lines are being tested in multiple locations in 2011. Also, more than 8,000 seeds were advanced by growing them in Puerto Rico during the winter. These seeds being grown in Wooster in 2011 for selection of single plants with high-protein (>44%) and desirable agronomic traits.
During the three year period of this grant (2008-11), more than 25 cross combinations were made by crossing elite Ohio cultivars with advanced breeding lines with high proteins from other collaborators. The progeny lines were advanced to F5 generation by single seed descend (SSD) and then evaluated for high-protein in Ohio. The others are still being advanced to be field tested in 2011 and 2012 under a new project/grant :3607-2100-012-12R. Besides, 57 plant introductions (PIs) with large variations in seed protein were evaluated in the field and 10 lines with more than 46% proteins were selected for crossing with elite Ohio lines.
This research relates to objective 4 (Develop soybean germplasm with high-yield and high seed-protein content) of the parent project.