DEVELOPMENT OF SOYBEAN WITH HIGH SEED PROTEIN, LOW PHYTATE, AND ENHANCED FEED EFFICIENCY
2008 Annual Report
1a.Objectives (from AD-416)
Improvement of soybean seed composition through incorporation of new genes from exotic germplasm and wide crosses. These funds will directly support the soybean project objective #4. The Milestone: release germplasm by 2011.
1b.Approach (from AD-416)
Northern elite (HC99-2846 and HC01-289 and HF02-005) - high-yielding Ohio lines by south high-protein (GSF-114 from Roger Boerma, N01-10974 from Tommy Carter, Danbaekong from South Korea) crosses will be made in 2007. HC-01-289 is a MG-III high-protein (44%) Ohio line resealed in 2006. The F1 and F2 generations will be grown in Puerto Rico to give single seed decent F3 seeds. We will grow F3 plants in Wooster in 2008, classify plants for maturity and harvest individual plants. Seed from each plant will be analyzed for protein and oil content, and lines with >46% protein will be selected for further testing. Seeds of selected lines will be increased in Puerto Rico. In 2009, we will test two row plots in a replicated trial and select lines with desirable agronomic traits and high-protein content. In 2010 and 2011, we will conduct multi-row replicated trial of selected lines with check cultivars in at least two locations. Seed yield, protein, and other agronomic traits will be measured, seeds will be increased, and the best lines will be released as germplasm. More crosses will be made as new sources of high-protein become available from collaborators (e.g., soja derived high-protein line from Istvan Rajcan Canada).
F3:4 lines from crosses of Ohio cultivars Wyandot (MG-II) and FG05 with SF-114 have been planted in Wooster, Ohio for a replicated yield trial. The protein contents of these lines are as high as 49% on a dry weight basis. The protein plus oil are as high as 67%. The yield trial will reveal the yield potentials of the high protein lines. Six more crosses were made in 2007 using three Ohio lines and two southern high-protein lines. N01-10974 (MG-VI) and S05-5451 (MG-IV) were crossed to three relatively high-protein (40 - 44%) cultivars - 'Wyandot' (MG-II), 'Wooster' (MG-III) and 'Prohio' (MG-IV). We will have F4 (SSD) lines from these crosses in the Fall. Two hundred plant introductions were tested for high-protein and/or high-oil contents. We have identified several lines that do not show the negative relationship between protein and oil contents. These PIs should be of interest for studying the protein oil relationship in soybean. Next Quarter Activities:
Make six new crosses for high protein and protein plus oil. Advance SSD lines for six crosses from 2007.