2010 Annual Report
1a.Objectives (from AD-416)
Improvement of soybean seed composition through incorporation of new genes from exotic germplasm and wide crosses. These funds will directly support the soybean project objective #4. The Milestone: release germplasm by 2011.
1b.Approach (from AD-416)
North elite (HC99-2846 and HC01-289 and HF02-005 - high-yielding Ohio lines) by south high-protein (GSF-114, N01-10974, from South Korea) crosses will be made in 2007. HC-01-289 is a MG-III high-protein (44%) Ohio line resealed in 2006. The F1 and F2 generations will be grown in Puerto Rico to give single seed decent F3 seeds. We will grow F3 plants in Wooster in 2008, classify plants for maturity and harvest individual plants. Seed from each plant will be analyzed for protein and oil content, and lines with >46% protein will be selected for further testing. Seeds of selected lines will be increased in Puerto Rico. In 2009, we will test two row plots in a replicated trial and select lines with desirable agronomic traits and high-protein content. In 2010 and 2011, we will conduct multi-row replicated trial of selected lines with check cultivars in at least two locations. Seed yield, protein, and other agronomic traits will be measured, seeds will be increased, and the best lines will be released as germplasm. More crosses will be made as new sources of high-protein become available from collaborators (e.g., soja derived high-protein line from Canada).
48 F2:4 lines from crosses of two food-grade high-yielding Ohio cultivars -Wyandot (MG-II) and FG05 - with GASF-114 were planted in Wooster, Ohio for a two replication yield trial 2008. The protein contents of these lines are as high as 49% on a dry weight basis. The protein plus oil are as high as 66%. 20 F5 plants were pulled from border of 20 higher yielding lines for developing pure lines, because these lines were still segregating for phenotypes. The yield levels of several selected lines were comparable to the Ohio parent. Data from 400 single plants show 50 lines with >45% protein. We have planted single row plots of the high-protein lines (F5:6) in 2009 and have a good stand. 300 single-row plots were harvested, and 100 plots were abandoned because of poor phenotypes, disease susceptibility, etc. Yield of more than 200 plots were higher than the Ohio parents. The protein and oil data from Peoria show that 100 lines had >45% protein and many of these lines had equal or greater yield than the Ohio parent FG05. 10 lines with high-protein and high-yield were sent to Puerto Rico for seed increase. Multi-location replicated yield trials for these lines will be conducted in 2010. Four- replicate yield trials have been planted at four locations in Ohio with the seeds of the 10 selected high-protein lines along with proper check varieties.