2009 Annual Report
Objective 2: Develop agronomically-superior sugarbeet germplasm with resistance to Beet severe curly top virus (BSCTV).
Objective 3: Determine the distribution and diversity of sugarbeet root rot organisms.
Objective 4: Determine the effects of pathogens on sugarbeet post-harvest quality and storability.
Objective 5: Develop innovative disease management options for BSCTV and root rot organisms.
Paired crosses were made between resistant and susceptible sugarbeet parents to study inheritance of curly top. To augment the conventional hybridization (crossing) processes, tissue culture technology was utilized to develop pure lines. New haploid pure lines were produced from curly top resistant and susceptible elite lines. Doubling of the chromosomes, by using chromosomal doubling chemicals, is under progress. These inbred lines will be used as parental lines for developing mapping populations to identify genes regulating curly top. These lines and other progenies under development were genotyped with available DNA markers.
Objective 2. Germplasm screening for response to various important sugarbeet diseases is a continuous process to search for novel sources of genetic resistance. New plant introductions from cultivated sugarbeet and wild sugarbeet were screened in the curly top and rhizomania nurseries.
The survey of the curly top species in the Western U.S. has been published. To support the genetics work, scientists have also been cooperating on a project to develop an enzyme-linked immune-sorbent assay (ELISA) that will quantify the amount of virus in the plant tissue. We are in the testing phase for this assay.
Objective 3. The survey of the fungi associated with root rot in the Amalgamated Sugar Company production area in southern Idaho has been accepted for publication. The Rhizoctonia isolates have been sequenced and submitted for publication along with the pathogenicity data collected the previous year. The Verticillium isolates were collected for a second year and placed in storage for future research. The Leuconostoc root rot assay research was repeated and submitted for publication.
Objective 4. The second year of determining storability of sugarbeet cultivars was completed, and data from the project were published. A preliminary study was conducted to identify the influence of roots with bacterial root rot on the storability of healthy roots in an outdoor pile.
Objective 5. Rhizoctonia/manure studies were repeated and the pathogenicity portion of the data was published with the fungal survey data. The isolates for the microbiology portion of this project are currently being identified through sequencing, Biolog plates, and other laboratory tests. Two seed treatment trials were conducted to test the efficacy of seed treatments and host resistance in controlling curly top and pests in sugarbeet.
Eujayl, I.A., Morris, C.F. 2009. Identification of differentially expressed UniGenes in developing wheat seed using digital differential display. Journal of Cereal Science. 49:316-318.
Strausbaugh, C.A., Wintermantel, W.M., Gillen, A.M., Eujayl, I.A. 2008. Curly top survey in the Western United States. Phytopathology. 98(11):1212-1217.
Strausbaugh, C.A., Eujayl, I.A., Rearick, E., Foote, P., Elison, D. 2009. Sugar beet cultivar evaluation for storability and rhizomania resistance. Plant Disease. 93:632-638.
Gillen, A.M., Strausbaugh, C.A., Tindall, K.V. 2008. Evaluations of Beta corolliflora for Resistance to Curly Top in Idaho. Journal of Sugar Beet Research. 45(3&4):99-118
Eujayl, I.A., Strausbaugh, C.A. 2009. Evaluation of sugar beet germplasm and plant introductions response to rhizomania and storability in Idaho, 2008. Plant Disease Management Reports. 3:FC105.
Strausbaugh, C.A., Eujayl, I.A., Rearick, E., Foote, P. 2009. Experimental sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2008. Plant Disease Management Reports. 3:FC107.
Strausbaugh, C.A., Eujayl, I.A., Rearick, E., Foote, P. 2009. Commercial sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2008. Plant Disease Management Reports. 3:FC108.