2011 Annual Report
1a.Objectives (from AD-416)
Develop public soybean germplasm containing less than 3% linolenic acid in the oil fraction in three unique platforms for a range of maturity groups.
1b.Approach (from AD-416)
1. Make nine crosses and develop initial populations segregating for GmFAD3A and GmFAD3C alleles. 2. Use molecular markers to select desired genotypes. 3. Backcross selected genotypes and develop segregating populations. 4. Use molecular markers to select desired genotypes in 75% recurrent parent genome. 5. Advance material identified with desired genotypes. 6. Evaluate fatty acid profile of selected lines.
The overall objective of this project was to create new sources of less than 3% linolenic acid soybean lines in three different maturity groups that could be used in breeding programs to incorporate the low lin oil phenotype. The objective links to the in-house project "To develop the molecular basis for modification of the fatty acid components of soybean oil and anti-nutritional components in soybean meal to use in accelerated breeding programs" by developing and testing soybean lines that contain new combinations of alleles that control the linolenic acid content in seed oil. The objective was achieved, with one new combination of the two genes responsible for less than 3% linolenic acid introgressed into the group lines representing maturity groups 1 (MG I), 3 (MG III), and 5 (MG V). In addition, we have a new combination of the two genes responsible for less than 3% linolenic acid advanced into a group 3 (MG III) background.
The low lin lines were confirmed to have relative maturity similar to the parental donors, and all produced seed in Columbia, Missouri with less than 3% linolenic acid in the seed oil. One MG III line appeared to have captured resistance to soybean cyst nematode from the Maverick parent.