2010 Annual Report 1a.Objectives (from AD-416) Develop public soybean germplasm containing less than 3% linolenic acid in the oil fraction in three unique platforms for a range of maturity groups. 1b.Approach (from AD-416) 1. Make nine crosses and develop initial populations segregating for GmFAD3A and GmFAD3C alleles. 2. Use molecular markers to select desired genotypes. 3. Backcross selected genotypes and develop segregating populations. 4. Use molecular markers to select desired genotypes in 75% recurrent parent genome. 5. Advance material identified with desired genotypes. 6. Evaluate fatty acid profile of selected lines. 3.Progress Report Research continued to develop low linolenic acid soybean germplasm in three different maturity groups. All three maturity groups now have the desired genes introgressed into 75% of the recurrent parent genome. The second low linolenic gene combination is being advanced as 50% of the maturity group III recurrent parent genome only due to earlier problems with crossing. All lines are stable for the desired genes controlling the low linolenic acid trait and are currently undergoing agronomic selection to identify potential lines for germplasm release. Monitoring activities included email exchange of information and results in quarterly reports, and participation in the United Soybean Board Soybean Seed Composition Workshop in February in St. Louis, Missouri.