2009 Annual Report 1a.Objectives (from AD-416) This Cooperative Agreement is for the purpose of carrying out cooperative research and development of control technologies (male annihilation) in an area-wide pest management program against fruit flies in Hawaii. The overall purpose of the project is to integrate technology developed by ARS and others in specific cropping areas of Hawaii to demonstrate that fruit fly control can lead to increased crop productivity, more environmentally acceptable control methods and economic benefit to those using the technology. The specific goals of the cooperative agreement are to integrate the SPLAT technology provided by ISCA Technologies of Riverside, California into the area-wide program which will facilitate a broader acceptance of the IPM technology, foster cooperative partnerships in research and education, and better serve the expanding diversified agricultural base of Hawaii and the Pacific. 1b.Approach (from AD-416) The work plan identifies the following major areas of study: 1. Research and development of male annihilation treatments that are environmentally sound and effective. 2. Registration with EPA of promising products. 3. Implementation of selected IPM technologies in Hawaii and California. Documents SCA with ISCA Technologies (Pest Management Tools & Solutions). 3.Progress Report Spinosad Replacement for Organophosphate Male Annihilation Treatments in California. Studies were conducted in Hawaii to quantify attraction and feeding response resulting in mortality of the male oriental fruit fly to a novel male annihilation treatment (MAT) formulation consisting of specialized pheromone and lure application technology (SPLAT) in combination with methyl eugenol (ME) and spinosad (=SPLAT-MAT-ME with spinosad) in comparison with Min-U-Gel ME with naled (Dibrom) currently used in California and Florida. Our approach involved a novel behavioral methodology for evaluation of slow-acting reduced-risk insecticides. Methyl eugenol treatments were weathered for 1.2.3.4, and 8 weeks in California and shipped to Hawaii for bioassays. Our results suggest that spinosad with low contact toxicity, and when mixed with SPLAT-ME offers a reduced-risk alternative for control of oriental fruit fly, without many of the negative effects to humans and non-targets of broad spectrum contact poisons such as naled. SPLAT-MAT-ME with spinosad offers potential for control of males in an area-wide integrated pest management (IPM) system without the need for conventional organophosphates. Progress is monitored through meetings with cooperator, telephone and email communications, and progress reporting.