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United States Department of Agriculture

Agricultural Research Service

Research Project: SOYBEAN PROTEIN IMPROVEMENT ACCELERATED BY PERFECT MOLECULAR MARKERS

Location: Plant Genetics Research

2011 Annual Report


1a.Objectives (from AD-416)
Evaluate the progress that can be achieved in developing soybean germplasm with improved meal value utilizing perfect molecular markers.


1b.Approach (from AD-416)
1. Make crosses and develop initial populations segregating for tractable meal traits. 2. Use molecular markers to select desired genotypes. 3. Backcross selected genotypes and develop segregating populations. 4. Advance material identified with desired genotypes. 5. Evaluate meal trait phenotypes and agronimic characteristics of lines.


3.Progress Report

The objective of this project was to determine efficient plant breeding strategies utilizing molecular markers to incorporate multiple seed quality traits into soybean lines with high yield potential. The objective links to the in-house project "To develop the molecular basis for modification of the fatty acid components of soybean oil and anti-nutritional components in soybean meal to use in accelerated breeding programs" by determining the molecular genetic basis for the low phytate trait and developing new knowledge about the strategy and population structures needed to incorporate seven genes in an accelerated plant breeding program. The original strategy was designed as a recurrent parent platform with a goal of combining seven independent genes controlling five different oil and meal traits. The recurrent parent was also intended to be a low phytate (two genes) trait donor in addition to supplying favorable yield potential and appropriate maturity, in this case a maturity group 5 (MG V) line.

Several factors limited the success of this project in terms of the germplasm produced, although the objective of evaluating molecular marker assisted plant breeding strategies was achieved. Despite these issues, we were able to utilize molecular marker assays in the original and then the modified plant breeding strategy to combine multiple seed composition traits together in one line. We determined that it is possible to bring together four traits in four years using the MG V recurrent parent strategy; with a MG IV or earlier maturing line as the recurrent parent, at least one year could be saved and more traits could likely be combined efficiently. We did not have the opportunity to evaluate any of the developed lines for yield.


Last Modified: 11/27/2014
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