1b.Approach (from AD-416):
Association mapping in elite varieties.
Beta glucan is a compound that has been shown to promote heart and vascular health. Oats are an excellent source of beta glucans. In the lifetime of this project, we developed a new method for measuring beta glucan content of oats. This method reduces costs by 90 percent and requires five percent of the time required by other methods, while still producing results that are similar to a standard method. In addition, we extracted DNA that was used for genotyping oat varieties. Cooperators on this project used genotype information in conjunction with beta glucan values, determined using our new method, to carry out genome wide association studies and genomic selection for beta glucan content. As a result, we identified genetic loci involved in determining beta glucan content and developed and released new oat varieties with increased content of beta glucan.