DEVELOPMENT OF GRASS GENOMIC RESOURCES FOR CROP IMPROVEMENT AND BIOFUEL RESEARCH
Genomics and Gene Discovery
2013 Annual Report
1a.Objectives (from AD-416):
Grass species including many important cereal and bioenergy crops are integral to U.S. future plans for sustainable food and energy. To meet the increasing demands for food and energy fuel worldwide, it is necessary to develop valuable genomics resource and knowledge that can be directly applied to crop improvement and biofuel research. This project will use genomics and bioinformatics to develop knowledge and resources for cereal and biofuel improvement, including utilizing the new grass model species Brachypodium to search for genes associated with agronomical traits such as grain development and cell wall biosynthesis. Project will enhance data within public databases and connect these data to other relevant data and databases.
1b.Approach (from AD-416):
1) Improve Agrobacterium-mediated transformation of Brachypodium for high-throughput generation of T-DNA tagged mutant population.
2)Contribute to the development, maintenance, and distribution of T-DNA tagged mutant plants.
3)Molecular and genetic characterization of mutants by directly linking the corresponding genes onto genetic and physical maps of both wheat and Brachypodium, .
4)Collaborate in development software tools for processing of data and website to make the data accessible to public..
5)Data collation and curation for inclusion in the GrainGenes database. Documents SCA with UC Davis.
Brachypodium is a simple model for studying the unique properties of the grasses and has been adopted by many laboratories around the world because of its ease of use and long list of readily available resources. This work relates to the parent project by providing experimental resources, T-DNA tagged mutants, that are used to achieve the objectives of the parent project more rapidly. In FY13 we created over 5,000 T-DNA lines bringing the total T-DNA collection to about 20,000 lines. We sequenced the DNA flanking the insertion sites in over 10,000 lines in FY13 (18,000 total) in order to determine which genes are inactivated by T-DNA insertions. These insertion sites were incorporated into the project database. Over 600 lines were requested through the project website in FY13 (>1,600 total). This resource will aid the determination of gene function which in turn will provide knowledge that can be used to create improved crops.