2010 Annual Report
Objective 1b. Seed increases of experimental strains of smooth bromegrass, western wheatgrass, tall wheatgrass, and two native legume germplasms have been increased for release pending official approval.
Objective 2a: Seed was produced in 2009 and blended with seed lots from previous years to provide adequate seed for pasture planting in autumn 2009. Weed control and adequate precipitation in spring and early summer 2010 promoted successful pasture establishment. Pastures will be fenced and grazed beginning in spring 2011.
Objective 2b: Field comparisons of the grassland assessment tool, elongated leaf height and canopy height were completed in bioenergy production systems. Work is still in progress in other grassland systems.
Objective 3a. A non-radiometric means to assay a key enzyme in the lignin biosynthesis pathway has been developed. This assay will prove useful to researchers working to understand and improve cell wall properties in feedstocks. Several antibodies were validated by immunoblotting and are ready for use in the protein array.
Objective 3b. A lowland type of switchgrass with excellent winter survival and disease tolerance is in accelerated seed increase for potential release. Switchgrass germplasms that are plant hardiness zone composite populations, populations bred for differences in tillering potential, and differing in dry matter digestibility and lignin concentration have been prepared for release in CY10 pending official release approval.
Objective 4a. Sub-objective to develop new seed quality tests for big bluestem and indiangrass was deleted in 2010 because of reduced technical support.
Objective 4b: Results from a large scale switchgrass bale storage study are being analyzed. Study included big round and big square bales and various storage formats ranging from uncovered outside storage to dry indoor storage. To date, all 2,900 bale samples have been processed and are being analyzed for composition.
Objective 4c. A long-term switchgrass soil C sequestration study was established in eastern Nebraska 1998. The study includes two switchgrass cultivars, three N fertilizer rates and two harvest treatments. In the 9 year period from the spring of 1998 to the spring of 2007, soil C increased at rate of 2 Mg per hectare per year in plots in which best management practices were used. These results fully support switchgrass soil carbon sequestration data obtained previously obtained in a five-year study on ten farms in NE, SD, and ND.
Gutsche, A.R., Heng-Moss, T.M., Higley, L.G., Sarath, G., Mornhinweg, D.W. 2009. Physiological Responses of Resistant and Susceptible Barley, Hordeum vulgare to the Russian Wheat Aphid, Diurpahis noxia (Mordvilko). Arthropod-Plant Interactions. 3(4):233-240.
Smagghe, B.J., Hoy, J., Percifield, R., Hargrove, M.S., Sarath, G., Hilbert, J., Watts, R., Dennis, E., Peacock, J., Dewilde, S., Moens, L., Blouin, G., Olson, J.S., Appleby, C.A. 2009. Correlations between oxygen affinity and sequence classifications of plant hemoglobins. Biopolymers. 91(12):1083-1096.
Violante-Mota, F., Moran, J.F., Sarath, G., Areedondo-Peter, R. 2010. Analysis of the Peroxidase Activity of Rice (Oryza Sativa) Recombinant Hemoglobin 1: Implications for the In Vivo Function of Hexacoordinate Non-Symbiotic Hemoglobins in Plants. Phytochemistry. 71(1):21-26.
Vogel, K.P., Mitchell, R.B., Gorz, H.J., Haskins, F.A., Newell, L.C., Klopfenstein, T.J., Erickson, G., Anderson, B.E. 2010. Registration of ‘Warrior’, ‘Scout’, and ‘Chief’ Indiangrass. Journal of Crop Registrations. 4:115-122.
Vogel, K.P., Mitchell, R.B., Baltensperger, D.D., Johnson, K.D., Carlson, I.T. 2010. Registration of 'Homestead' Canada Wildrye. Journal of Crop Registrations. 4:123-126.
Gulsen, O., Eickhoff, T., Heng-Moss, T., Shearman, R., Baxendale, F., Sarath, G., Lee, D., Nabity, P. 2010. Characterization of Peroxidase Changes in Resistant and Susceptible Warm- Season Turfgrasses Challenged by Blissus Occiduus. Arthropod-Plant Interactions. Vol 4: 45-55
Li, C., Knierim, B., Manisseri, C., Arora, R., Scheller, H.V., Auer, M., Vogel, K.P., Simmons, B., Singh, S. 2009. Comparison of Dilute Acid and Ionic Liquid Pretreatment of Switchgrass: Biomass Recalcitrance, Delignification and Enzymatic Saccharification. Bioresource Technology. 101:4900-4906. doi:10.1016/j.biortech.2009.10.066.
Dien, B.S., Sarath, G., Pedersen, J.F., Sattler, S.E., Chen, H., Funnell-Harris, D.L., Nichols, N.N., Cotta, M.A. 2009. Improved Sugar Conversion and Ethanol Yield for Forage Sorghum (Sorghum bicolor L. Moench) Lines with Reduced Lignin Contents. BioEnergy Research. 2(3):153-164.
Qureshi, N., Saha, B.C., Hector, R.E., Dien, B., Hughes, S., Liu, S., Iten, L., Bowman, M.J., Sarath, G., Cotta, M.A. 2010. Production of butanol (a Biofuel) from agricultural residues: Part II - Use of corn stover and switchgrass hydrolysates. Biomass and Bioenergy. 34(4):566-571.
Adler, P.R., Sanderson, M.A., Weimer, P.J., Vogel, K.P. 2009. Plant species composition and biofuel yields of conservation grasslands. Ecological Applications. 19(8):2202-2209.
Schmer, M.R., Mitchell, R., Vogel, K.P., Schacht, W.H., Marx, D.A. 2010. Spatial and Temporal Effects on Switchgrass Stands and Yield in the Great Plains. BioEnergy Research. 3:159–171.
Arora, Rohit, Manisseri, Chithra, Li, Chenlin, Ong, Marcus, Scheller, Henrik Vibe, Vogel, Kenneth, Simmons, B.A., Singh, Seema. 2010. Monitoring and Analyzing Process Streams Towards Understanding Ionic Liquid Pretreatment of Switchgrass (Panicum virgatum L.). BioEnergy Research. 3:134-145.
Palmer, N.A., Sattler, S.E., Saathoff, A.J., Sarath, G. 2010. A Continuous, Quantitative Fluorescent Assay for Plant Caffeic acid O-Methyltransferases. Journal of Agricultural and Food Chemistry. 58: 5220-5226
Schmer, M.R., Mitchell, R., Vogel, K.P., Schacht, W.H., Marx, D.B. 2010. Efficient Methods of Estimating Switchgrass Biomass Supplies. BioEnergy Research. 3(3):243-250.