Location: Genomics and Gene Discovery
Project Number: 5325-21000-017-03
Start Date: Aug 01, 2007
End Date: May 31, 2012
Using an Agrobacterium-mediated transformation procedure we will create a collection of insertional mutants in the model grass Brachypodium distachyon. To determine what gene is disrupted in each mutant, we will sequence DNA flanking the insertion site using the known sequence of the inserted DNA as a starting point. This collection will then be used to identify mutations in genes predicted to affect biomass quality and agronomic characteristics of energy crops. The mutant collection will be made publicly available through a BLAST searchable database to allow other researchers to identify knockouts in specific genes.