1a.Objectives (from AD-416):
Develop strategies that promote cell proliferation and replacement in the bovine mammary gland, in order to increase lactational productivity and efficiency.
1b.Approach (from AD-416):
We propose to accomplish the research objective by utilizing and extending current knowledge of mammary stem cell biology. Our specific aims are to manipulate mammary stem cell activity to enhance the growth of mammary epithelium and promote the replacement of senescent cells.
Experiments addressing Specific Aim #1 and 3 of the grant have been completed and a manuscript is in preparation. Research has identified potential mammary stem cell markers and the findings have been published. Treatment of mammary glands with inosine during the dry period (Specific Aim #3) did not increase milk production in the next lactation.