1a.Objectives (from AD-416)
1. Association mapping of beta-glucan content and in elite oat germplasm and replicated comparison of phenotypic with marker-assisted selection.
2. Association mapping of beta-glucan content from the National Plant Germplasm System: complementation of elite oat.
3. Educational initiatives to pipeline students into plant breeding and to educate professionals.
1b.Approach (from AD-416)
Apply association analysis to a population of elite oat lines tested. 200 lines from each tail of the distribution to genotypewith the marker system will be picked. Data for association alaysis of barley beta-glucan content will be obtained from the barley CAP and analyzed, providing a first source of candidate sequences. An Interactive distance short course will be developed.
Two Ph.D. students have been hired under this project. Seed of Germplasm has been obtained from the past ten years of the Uniform Oat Performance Nursery (UOPN) and from the National Plant Germplasm System (NPGS). Single reference plants from the UOPN germplasm have been grown and harvested for DNA extraction. The DNA has been sent to Diversity Arrays Technology for genotyping. We now have a large dataset of 1295 marker scores on each of the 462 entries. These data have been used to calculate kinships among all entries and has been reported in a presentation at the International Oat Conference in July 2008. We have collected data reports from these years of the UOPN and, with collaboration from Albany, CA Genomics and Gene Discovery Research Unit, these data reports have been entered into the GrainGenes database. They are now publicly available and the database will also serve our data analysis needs. Germplasm from NPGS has been planted in the field for observation of its adaptation prior to deciding which entries we will select for DNA extraction and marker genotyping. This progress addresses NP 301 Action Plan Component 3, Statement A.