2012 Annual Report 1a.Objectives (from AD-416): Identify compounds in potatoes that have nutritional or health-promoting value. 1b.Approach (from AD-416): Diverse potato germplasm will be analyzed by LCMS to determine phytonutrient content. Potato extracts will be tested for anti-cancer activity using human cell cultures. 3.Progress Report: This is the final report for this project. Potato germplasm was evaluated for phytochemical content and tested for anti-cancer activity. In prostate cancer cells, potato extracts exhibited both anti-proliferative and pro-apoptotic activity, which is covered under objective 3.A. of the related in-house project, "Elucidate genetic, molecular and biochemical processes governing accumulation of select phytochemicals and vitamins with respect to improving potato". Based on metabolite profiles and/or cell culture results, a purple-fleshed breeding line (CO112F2-2P/P) and an accession of S. bulbocastanum (PI243510) were advanced to a mouse model. Mice received xenografts of human prostate cancer cells and were administered extracts of potato phenolics. No significant reduction in tumor volume was observed. However, cell proliferation was reduced by half in both treatment groups and there was minimal damage to vital organs, suggesting that further studies are worthwhile. This study is currently being repeated using a higher dosage of potato phenolics, with the goal of inducing a large scale reduction in tumor volume.