1a.Objectives (from AD-416):
1. To develop elite clones of creeping bentgrass, with multiple disease resistances. 2. To deliver these clones to private bentgrass breeders for use in developing new cultivars. 3. To conduct DNA marker selection experiments, testing the hypotheses that selection for DNA markers can be used to identify disease-resistant plants of creeping bentgrass. 4. To identify the genomic source of each linkage group (chromosome).
1b.Approach (from AD-416):
Maintenance of high-quality bentgrass fairways and putting greens is a stressful and expensive task, one that could be simplified by genetic resistance to diseases. Four bentgrass breeding programs will join together into a consortium with the direct objective to support the golf industry with improved clones of creeping bentgrass and creeping-colonial hybrids. Clones will be selected for resistance to dollar spot and snow mold fungi, using molecular markers identified by research partially sponsored by USGA. Elite clones with combined resistance to dollar spot and snow mold will be identified and made available to private bentgrass breeders. Clones will be released on contractual arrangements for future royalty payments to the participating institutions and USGA.
These results support the objectives of the parent project by providing a mechanism to deliver new and improved germplasm to private breeders who are in a position to develop new cultivars in support of public and industry stakeholders. Creeping x colonial bentgrass mapping populations were evaluated for snow mold and dollar spot resistances at two locations in 2010-11. DNA marker analyses of progenies were conducted on all bentgrass crosses and quantitative trait locus (QTL) analyses will be conducted later in FY12. Preliminary analyses revealed two QTL involved in dollar spot resistance and three involved in snow mold resistance. These loci have been mapped to general regions of four chromosomes and nearby markers have been identified to allow researchers to conduct marker-based selection for these two traits.