1a.Objectives (from AD-416)
To identify genes associated with viral disease resistance in Carp.
1b.Approach (from AD-416)
Candidate immune response genes will be added on to a genetic linkage map that will contain QTL for viral disease resistance in carp.
The common carp is the most important farmed freshwater fish species in the world. Ornamental carp (koi) is an important product in both the US and Israel. Carp industries have recently suffered enormous economic damage due to a viral disease caused by Cyprinid herpes virus 3 (CyHV-3). Mapping and identification of candidate genes for viral disease resistance in carp provides insight to the underlying mechanisms of viral resistance in cool and cold water aquaculture species. In this project we identified and developed approximately 100 single nucleotide polymorphism (SNP) markers and genotyped a common carp reference mapping family using 23 informative SNPs from 14 genes known to be involved in mammalian innate immunity. SNPs from one gene, Interleukin 10a (IL-10a) were found to be associated with CyHV-3 resistance in the reference mapping family. In addition, we isolated full-length transcripts of the carp toll-like receptor 9 (TLR9) and two duplicated genes from its signaling pathway and characterized their expression profile in common carp and koi.