1a.Objectives (from AD-416)
To identify genes associated with viral disease resistance in Carp.
1b.Approach (from AD-416)
Candidate immune response genes will be added on to a genetic linkage map that will contain QTL for viral disease resistance in carp.
The common carp is the most important farmed freshwater fish species in the world. Ornamental carp (koi) is an important product in both the US and Israel. Carp industries have recently suffered enormous economic damage due to a viral disease caused by Cyprinid herpes virus 3 (CyHV-3). Mapping and identification of candidate genes for viral disease resistance in carp will provide insight to the underlying mechanisms of viral resistance in cool and cold water aquaculture species. In collaboration with scientists from The Hebrew University-Israel and Virginia Tech University, we identified 183 putative single nucleotide polymorphisms (SNP) in 10 genes known to play an important role in the mammalian immune response to viral diseases and developed 45 SNP markers for genetic mapping. These markers will be used in future genetic analyses to determine if they are associated with variations in immune response and resistance to CyHV-3 in carp. The ADODR is in frequent contact with the cooperator through phone calls, email, and annual site visits in addition to receipt of written reports.