2013 Annual Report
Objective 2: Develop enhanced potato varieties that benefit U.S. potato producers and consumers, including russets (fresh market and processing), long whites (processing), round whites (chipping), and specialty (red-skinned and yellow-fleshed), with emphasis on disease and pest resistance, reduced sugar accumulation, reduced need for production inputs, and enhanced nutritional qualities.
Objective 3: Develop marker-assisted selection (MAS) protocols for potato traits, with an emphasis on pathogen and pest resistances.
Objective 4: Characterize foliar and tuber responses of potato varieties to infection by newly identified strains of potato virus Y (PVY), and identify new sources of resistances to these PVY strains useful to potato breeders.
Substantial results were realized over the 5 year duration of this project, with progress made on all four project objectives. Eleven potato varieties were developed and released with collaborators of the Northwest (Tri-State) Potato Variety Development Program which contributed to Objective 2 of the project that emphasizes the development of enhanced potato varieties. Eight of the eleven potato varieties originated from this project’s hybridization program, with University of Idaho colleagues and this project leading in the release of the varieties Classic Russet, Alpine Russet, Clearwater Russet, Palisade Russet, Teton Russet, and Huckleberry Gold. Characteristics of each variety have been previously reported in this project’s FY08 to FY12 annual reports and with publications in the American Journal of Potato Research. Significant progress also was made in Objective 3, with the development and use in our project of molecular markers linked to extreme resistance to potato virus Y (PVY). In addition, in collaboration with University of Idaho researcher further fine-mapping of potato leafroll virus (PLRV) resistance conferred by the Rlretb gene identified by our project was conducted. Molecular markers more close linked to t Rlretb and useful for marker-assisted selection (MAS) were identified. Field evaluations of a family segregating for traits were also conducted as part of a national research effort termed “SolCAP” to link genes important in carbohydrate and vitamin biosynthesis in potato with molecular markers useful for MAS. A subset of these SolCAP breeding clones are now being used for identifying molecular markers important in the formation of acrylamide, with this project supplying seed and being one of two field test sites. This project also contributed advanced breeding clones and varieties for the National Fry Processing Trial (NFPT) which seeks to identify processing varieties having low acrylamide that could be rapidly adopted by the U.S. potato processing industry, and also oversaw a NFPT trial site for the last three years. As part of the SCRI Zebra Chip (ZC) Research initiative, potato germplasm from this project was identified as having resistance to ZC disease and its insect vector. This is notable in that potato varieties currently utilized by industry have shown no or little resistance to ZC with this research contributing to project objective 1. Our project also manages a trial site in Idaho that is screening U.S. germplasm for resistance to PVY in collaboration with researchers in New York and Wisconsin. In addition, our work to screen for PVY resistance in intra-genetically modified lines from collaborators in Michigan has shown that three previously PVY susceptible varieties now exhibit resistance to three PVY strains. This PVY research contributed to project objective 4.
Vales, M.I., Brown, C.R., Yilma, S., Hane, D.C., James, S.R., Shock, C.C., Charlton, B.A., Karaagac, E., Mosley, A.R., Culp, D., Feibert, E., Stark, J.C., Pavek, M.J., Knowles, N.R., Novy, R.G., Whitworth, J.L. 2012. Purple Pelisse: A specialty fingerling potato with purple skin and flesh and medium specific gravity. American Journal of Potato Research. 89:306-314.
Mc Cue, K.F., Ponciano, G.P., Rockhold, D.R., Whitworth, J.L., Gray, S.M., Fofanov, Y., Belknap, W.R. 2012. Generation of PVY coat protein siRNAs in transgenic potatoes resistant to PVY. American Journal of Potato Research. 89(5):374-383. DOI: 10.1007/s12230-012-9257-0.
Brown, C.R., Valves, M., Yilma, S., James, S., Hane, D., Shock, C., Feibert, E., Charlton, B., Culp, D., Pavek, M., Knowles, R., Novy, R.G., Whitworth, J.L., Stark, J., Miller, J., Holm, D., Navarre, D.A. 2012. “AmaRosa,” a red skinned, red fleshed fingerling with high phytonutrient value. American Journal of Potato Research. 89:249-254. DOI 10.1007/s12230-012-9248-1.
Brown, C.R., Haynes, K.G., Moore, M., Pavek, M.J., Hane, D.C., Love, S.L., Novy, R.G., Miller, J. 2012. Stability and broad-sense heritability of mineral content in potato: calcium and magnesium. American Journal of Potato Research. 89:255-261. DOI 10.1007/s12230-012-9240-9.