Location: Foreign Animal Disease Research
Project Number: 1940-32000-051-00
Start Date: Aug 01, 2007
End Date: Dec 31, 2008
Real-time PCR tests for Rinderpest and Capripoxviruses were developed under the previous project (CRIS 1940-32000-041-00X). Under this project we will complete the bench validation of these tests utilizing laboratory generated samples as well as those from experimentally infected animals. Tests will be evaluated for clinical sensitivity and specificity. Test performance will be evaluated and final reports prepared and submitted to APHIS for field validation. A real-time RT-CR for rapid detection of VSV has been previously developed that is capable of detecting all currently known genetic lineages of VSV. This test has been thoroughly evaluated in experimental and clinical samples and has been extensively utilized by APHIS during recent outbreaks in the southwestern USA. Since VSV is insect transmitted it is now necessary to modify and evaluate this test for detection of VSV in insects. Insects inoculated with VSV will be used for RNA extraction. Various RNA extraction methods will be tested and quality of RNA evaluated. RNA samples will be tested utilizing current real-time PCR.