APPLICATION OF PLANT-VIRAL BASED VECTORS TO THE DEVELOPMENT OF NOVEL DISEASE CONTROL STRATEGIES
Project Number: 1275-22000-252-00
Start Date: Jun 25, 2007
End Date: Mar 15, 2012
Objective 1: Characterize plant viral genomes and develop novel plant virus-based vectors for expression of foreign gene sequences in plants.
Objective 2: Evaluate plant and pathogen gene function in host/pathogen interactions and disease resistance (functional genomics), and candidate sequences for plant disease control.
Objective 3: Develop new practical strategies for the production of vaccines and other biomedical products in plants for prevention, treatment, and control of animal diseases.
In Objective 1, we will 1) develop novel plant virus-based gene expression vectors, and 2) develop novel vector strategies based on foreign gene expression from defective interfering RNAs and subgenomic RNAs.
In Objective 2, we will 1) develop virus-induced gene silencing to suppress pathogenic and disease-related genes of cellular and subcellular pathogens, 2) determine the role of plant protein phosphorylation signaling pathways in host/pathogen interactions, and 3) evaluate genes with potential for conferring resistance to cellular and subcellular pathogens and modulation of biochemical pathways involved in host/pathogen interactions and innate immunity.
In Objective 3, we will 1)test the ability of an epitope presentation system based on Cucumber Mosaic Virus coat protein expressed from a Potato Virus X vector to produce novel vaccines and applications for nanotechnology, 2) develop a new strategy for treatment and control of coliform mastitis in dairy cows by utilizing a plant-manufactured antimicrobial agent, 3) develop stable transient expression modules for production of useful biomedical products in plants, and 4) develop multi-component vaccines and diagnostic reagents by expression of multiple foreign epitopes on the surface of a plant virus-like particle.