Project Number: 5320-22430-025-12
Project Type: Specific Cooperative Agreement

Start Date: Sep 20, 2007
End Date: Sep 19, 2012

Objective:
1) Identify the active components of the sex pheromone for O. sacchari and E. Fullonia, 2) Synthesize the pheromone and validate attractiveness in lab and field, 3) determine seasonal pattern of O. sacchari and E. Fullonia in agricultural settings, and 4) determine optimal timing of control based on pheromone-based trapping data.

Approach:
Our approach is to collect authentic pheromone from live ‘calling’ females and/or clipped abdominal tips and using headspace collection and or tip extraction to identify the chemicals present using GC-MS. Electrophysiological studies will be employed to identify active constituents using GC-EAD on male moth antenna. Those with activity will be tested and formulated for activity against the putative pheromone in a flight tunnel bioassay and if possible in the field. Next the pheromone will be synthesized and pheromonal-based lures used to conduct field evaluations. Specifically we would like to correlate moth capture to field populations and damage levels in the different cropping systems unique to Hawaii. Lastly we hope to use pheromone trap capture as a predictive tool to assess the timing of control tactics. Documents SCA with Eastern Mennonite University. Formerly 5320-22430-021-13S (11/10).