2010 Annual Report 1a.Objectives (from AD-416) To identify and differentiate typical and atypical case samples of CWD and Scrapie by characterizing the biologic phenotype and pathologic profile of these agents when administered to susceptible lines of transgenic and inbred mice. 1b.Approach (from AD-416) Tissue samples from deer, elk, sheep and goats with Transmissible Spongiform Encephalopathy (TSE) will be administered to mice by intracerebral injection. Multiple tissue types will be included, such as samples of brain, lymph node, blood, urine, feces, antler velvet and muscle. Mouse models used as recipient hosts will include both preexisting and recently created transgenic and inbred mouse lines. Recipient mouse phenotype will be evaluated by measuring clinical response, population disease rate, incubation time, and pathologic profile within the central nervous system (CNS). Pathologic profile of CNS lesion foci is assessed by evaluating anatomic localization, spongiform change, astrocytic gliosis, and deposition of protease resistant prion protein. Documents SCA with U. of WA. 3.Progress Report The purpose of this SCA is to identify and differentiate typical and atypical case samples of Chronic Wasting Disease (CWD) and scrapie by characterizing the biologic phenotype and pathologic profile of these agents when administered to susceptible lines of transgenic and inbred mice. Mice have been inoculated with tissue from sheep with two Nor98-like isolates and classical scrapie, and with tissue from mule deer. Mice are monitored for incubation time and clinical disease, and evaluated postmortem for pathologic changes in the brain and other tissues along with accumulation of abnormal prion within the tissues. There are regular interactions between the ADODR, ADRU scientists and University of Washington collaborators through personnel visits, conference calls and electronic exchange of data.