Location: Cereal Disease Laboratory
2011 Annual Report
Objective 1: Monitor and characterize races of cereal rust pathogens, particularly the new East African strain, Ug99. This includes the sub-objective of characterizing races of cereal stem rust, particularly Ug99 and related mutants, which represent a threat to the production of wheat, oat, and barley cultivars in the United States.
Objective 2: Identify features essential for cereal rust pathogensis including sub-objectives of characterizing the genome of Puccinia graminis (stem rust). Research for this objective will be expanded to characterize the Ug99 genome sequence and to develop detection methods for the new Eastern African stem rust mutants.
Objective 3: Identify resistance genes and develop effective strategies for deploying host-resistance genes to control cereal rust diseases. Research for this objective will be expanded to identify new sources of Ug99 resistance and to accelerate the development of Ug99-resistant wheat and barley varieties adapted for U.S. production.
Potential sources of effective, new crown rust resistance genes were found among 338 accessions of tetraploid and diploid wild Avena species from Morocco. A new statistical approach to identifying potential sources of partial resistance to oat crown rust was tested using data from the Saint Paul oat/buckthorn crown rust nursery and appears promising. Mapping of partial resistance to crown rust in the PI260616/Otana RI population has revealed new resistance QTL.
Genetic analysis was conducted to determine the nature of resistance to wheat leaf rust pathogen in two wheat lines. A wheat line derived from a Uruguay landrace was shown to contain two resistance genes, one of which is likely a new wheat leaf rust resistance gene. The adult plant resistance in a line of winter wheat developed at Purdue University is likely made up of two previously described leaf rust resistance genes.
A new assay was developed that is able to distinguish between the different members of the wheat stem rust pathogen Ug99 race group from Africa. Methods were developed for genotyping field samples of killed wheat stem rust pathogen, which enables rapid monitoring for this pathogen on a global scale. Pilot project was completed with samples from Africa and Central Asia. New molecular assays were developed from rapid identification of rust fungi that are common pathogens of corn, and native prairie grasses that are being developed for biofuel production.
Carson, M.L. 2010. Additional sources of broad-spectrum resistance to Puccinia coronata f. sp. avenae in Canadian accessions of Avena barbata. Plant Disease. 94:1405-1410.
Zhang, W., Olson, E., Saintenac, C., Rouse, M., Abate, Z., Jin, Y., Akhunov, E., Pumphrey, M., Dubcovsky, J. 2010. Genetic maps of stem rust resistance gene Sr35 in diploid and hexaploid wheat. Crop Science. 50(6):2464-2474.
Njau, P.N., Jin, Y., Huerta-Espino, J., Keller, B., Singh, R. 2010. Identification and evaluation of new sources of resistance to stem rust race Ug99 in wheat. Plant Disease. 94:413-419.
Crouch, J., Szabo, L.J. 2011. Real-time PCR discrimination of the southern and common corn rust pathogens Puccinia polysora and P. sorghi. Plant Disease. 95(6):624-632.
Duplessis, S., Cuomo, C.A., Lin, Y., Aerts, A., Tisserant, E., Veneault-Fourrey, C., Joly, D., Hacquard, S., Amselem, J., Cantarel, B., Chiu, R., Couthinho, P., Feau, N., Field, M., Frey, P., Gelhaye, E., Goldberg, J., Grabherr, M., Kodira, C., Kohler, A., Kues, U., Lindquist, E., Lucas, S., Mauceli, E., Morin, E., Murat, C., Pearson, M., Quesneville, H., Rouhier, N., Sakthikumar, S., Schmutz, J., Selles, B., Shapiro, H., Tangay, P., Tuskan, G.A., Van De Peer, Y., Henrissat, B., Rouze, P., Schein, J., Dodds, P.N., Zhong, S., Hamelin, R.C., Birren, B.W., Grigoriev, I.V., Szabo, L.J., Martin, F. 2011. Obligate biotrophy features unraveled by the genomic analysis of the rust fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici. Phytopathology. 108:9166-9171.
Kolmer, J.A., Anderson, J.A. 2011. First detection in North America of virulence in Puccinia triticina to wheat seedlings with Lr21. Plant Disease. 95:1032.
Kolmer, J.A., Anderson, J.A., Flor, J.M. 2010. Chromosome Location, Linkage with Simple Sequence Repeat Markers, and Leaf Rust Resistance Conditioned by Gene Lr63 in Wheat. Crop Science. 50:2392–2395. Kolmer, J.A., Garvin, D.F., Jin, Y. 2011. Expression of a Thatcher wheat adult plant stem rust resistance QTL on chromosome arm 2BL is enhanced by Lr34. Crop Science. 51(2):526-533.
Kolmer, J.A., Long, D.L., Hughes, M.E. 2011. Physiologic specialization of Puccinia triticina on wheat in the United States in 2009. Plant Disease. 95:935-940.
Kolmer, J.A., Ordonez, M.E., Manisterski, J., Anikster, Y. 2011. Genetic differentiation of Puccinia triticina populations in the Middle East and genetic similarity with populations in Central Asia. Phytopathology. 101:870-877.
Niu, Z., Klindworth, D.L., Friesen, T.L., Chao, S., Jin, Y., Cai, X., Xu, S.S. 2011. Targeted introgression of a wheat stem rust resistance gene by DNA marker-assisted chromosome engineering genetics. Genetics. 187(4):1011-1021.
Qi, L.L., Pumphrey, M.O., Friebe, B., Zhang, P., Qian, C., Bowden, R.L., Rouse, M.N., Jin, Y., Gill, B.S. 2011. A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theoretical and Applied Genetics. 123:159-167.
Simons, K.J., Abate, Z., Chao, S., Zhang, W., Rouse, M., Jin, Y., Elias, E., Dubcovsky, J. 2011. Genetic mapping of stem rust resistance gene Sr13 in tetraploid wheat (Triticum turgidum subsp. durum L.). Theoretical and Applied Genetics. 122:649-658.
Tsilo, T., Jin, Y., Anderson, A. 2010. Identification of flanking markers for the stem rust resistance gene Sr6 in wheat. Crop Science. 50:1967-1970.
Liu, S., Yu, L., Singh, R., Jin, Y., Anderson, A. 2009. Diagnostic and co-dominant PCR markers for stem rust resistance genes Sr25 and Sr26. Theoretical and Applied Genetics. 120:691-697.
Ayliffe, M., Jin, Y., Kang, Z., Presson, M., Steffenson, B., Wang, S., Heung, H. 2011. Determining the basis of nonhost resistance in rice to cereal rusts. Euphytica. 179:33-40.
Glover, K.D., Rudd, J.C., Devkota, R.N., Hall, R.G., Jin, Y., Osborne, L.E., Ingemansen, J.A., Rickertsen, J.R., Hareland, G.A. 2011. Registration of "Select" Wheat. Journal of Plant Registrations. 5:196-201.
Jin, Y. 2011. Role of Berberis spp. as alternate hosts in generating new races of Puccinia graminis and P. striiformis. Euphytica. 179:105-108.
Liu, W., Pumphrey, M., Jin, Y., Rouse, M.N., Friebe, B., Gill, B. 2011. Development and characterization of wheat-Ae. searsii Robertsonian translocations and a recombinant chromosome conferring resistance to stem rust. Theoretical and Applied Genetics. 122:1537-1545.
Liu, W., Rouse, M.N., Friebe, B., Jin, Y., Gill, B., Pumphrey, M. 2011. Discovery and molecular mapping of a new gene conferring resistance to stem rust, Sr53, derived from Aegilops geniculata and characterization of spontaneous translocation stocks with reduced alien chromatin. Chromosome Research. 19(5):669-682.
Park, R.F., Fetch, T., Hodson, D., Jin, Y., Nazari, K., Pretorius, Z. 2011. International surveillance of wheat rust pathogens: progress and challenges. Euphytica. 179:109-117.
Rouse, M., Jin, Y. 2011. Stem rust resistance in A-genome diploid relatives of wheat. Plant Disease. 95:941-944.
Rouse, M.N., Wanyera, R., Njau, P., Jin, Y. 2011. Sources of resistance to stem rust race Ug99 in spring wheat germplasm. Plant Disease. 95:762-766.
Singh, R., Huerta-Espino, J., Bhavani, S., Herreta-Foessel, S., Singh, D., Singh, P., Velu, G., Mason, R., Jin, Y., Njau, P., Crossa, J. 2011. Race-nonspecific resistance to rust diseases in CIMMYT spring wheats (2010). Euphytica. 179:175-186.
Oliver, R.E., Lazo, G.R., Lutz, J.D., Rubenfield, M.J., Tinker, N.A., Anderson, J.M., Wisniewski-Morehead, N.H., Adhikary, D., Jellen, E.N., Maughan, P.J., Brown Guedira, G.L., Chao, S., Beattie, A.D., Carson, M.L., Rines, H.W., Obert, D.E., Bonman, J.M., Jackson, E.W. 2011. Model SNP development based on the complex oat genome using high-throughput 454 sequencing technology. Biomed Central (BMC) Genomics. 12:77.