Start Date: Feb 27, 2007
End Date: Feb 26, 2012
1A) Greenhouse and field trials will be conducted to improve the usefulness of nitrogenous amendments, such as biosolids, for managing plant-parasitic nematodes. Beneficial microbes, particularly species of Trichoderma and Pseudomonas, will be investigated for activity against nematodes and tested in the greenhouse and field for suppression of nematode populations. 1B) Studies will be conducted at the Farming Systems Project (FSP), Beltsville, MD, in different cropping systems to identify management practices which promote plant-parasitic nematode regulation and lead to development of suppressive soils. Soil nematodes with undetermined feeding habits will be studied to determine primary food sources in their native habitats. 2A) A diverse set of rye cultivars will be evaluated for M. incognita host status and benzoxazinoid content, cultivars will be tested in field trials for effects on nematode populatons on cotton and peanut, and fate of benzoxazinoids in soil will be determined. 2B) To identify nematotoxic activity from plant- and fungal-derived compounds, laboratory assays with root-knot nematodes will be conducted with various compounds, such as clove oil, fungal culture broth, and fescue root extracts. Promising compounds will be further tested in the greenhouse.