1a.Objectives (from AD-416)
To generate an integrated physical and genetic map for rainbow trout.
1b.Approach (from AD-416)
Use BAC DNA fingerprinting to construct a physical map and BAC end sequencing to identify genetic markers for integration of the physical and gentic maps.
This serves to document research conducted under a specific cooperative agreement between ARS and the University of California, Davis entitled, “Production of a physical map for the rainbow trout genome using high throughput DNA fingerprinting” and a reimbursable agreement between ARS and CSREES-NRI, entitled “Production of an integrated physical and genetic map for rainbow trout.” Over 100,000 bacterial artificial chromosome clones (5.5X genome coverage) were evaluated by DNA fingerprinting to initiate construction of a rainbow trout physical map to be integrated with the current genetic map. The ADODR is in frequent contact with the cooperator through phone calls, email, and annual site visits in addition to receipt of written reports.