1a.Objectives (from AD-416)
Identify the genetic map location of genes controlling pollen fertility restorer in sorghum. Identify potential candidate genes controlling pollen fertility in sorghum. Elucidate the mode of action of fertility restorer genes in controlling pollen viability in sorghum hybrids.
1b.Approach (from AD-416)
Utilize existing molecular marker technology to locate the position of fertility restorer loci on the annotated gene map of sorghum. Utilize the sequence of the sorghum genome to identify genes that belong to the pentatricopeptide gene family. Utilize bioinformatic tools to compare sequence of candidate fertility restorer genes to known fertility restorer genes in rice to more accurately predict functional fertility restorer genes and to better understand the mode of gene action in restoring pollen viability.
The goal of this project is to map and clone fertility restoration genes in sorghum, which are critical in hybrid sorghum production. During FY 2010, the pollen fertility restoration gene Rf5 was tentatively identified. This provides the required information to permit cloning of the complete set of fertility restoration genes, and will allow plant breeders to develop new female and male lines for hybrid seed production.
The ADODR of this project and the cooperator maintain regular communication by phone and e-mail, where progress of the work is discussed and evaluated, and where solutions to work impediments are developed. The ADODR and the cooperator meet periodically (at national scientific meetings, etc.) for face-to-face discussions of work direction and progress.