2009 Annual Report 1a.Objectives (from AD-416) This collaborative project between ARS (Gainesville, FL) and CEPAVE (LaPlata, Argentina) will evaluate trapping methods for surveillance and novel control methods for use against mosquitoes that vector diseases in Argentina, especially West Nile virus and dengue. 1b.Approach (from AD-416) ARS will provide trapping technology to include traps, methods and attractants for evaluation in Argentina. CEPAVE will conduct field trials with traps for surveillance of mosquitoes in Argentina. ARS will provide methods and compounds for control of mosquitoes including biological control agents and novel compositions. CEPAVE will conduct field studies to evaluate the effectiveness of control agents against natural mosquito populations. 3.Progress Report This project relates to the in-house project Objective 1: Develop new pathogen delivery formulations and molecular methods based on pathogenic viruses for control of mosquitoes; and Objective 3: Test and develop new and improved pesticides and "attract and kill" systems as management tools for pest and vector species. This is the second year of a project between ARS and researchers in Argentina. Argentine mosquitoes are distinct but because of similar climatic conditions there is a high potential for invasion and establishment which could negatively impact U.S. agriculture. This project will evaluate surveillance and control methods developed by ARS against Argentine mosquitoes to enhance remediation in the event of an invasion. This project could provide a model system for the protection of the U.S. from invasive mosquitoes in any part of the world. During this evaluation period, collaborative efforts have resulted in 1 publication on control of Argentine mosquitoes. ARS has provided collaborators in Argentina with molecular probes that will be used for screening mosquito populations for mosquito pathogenic baculoviruses. Progress was monitored through email correspondences and a meeting was held in June, 2009 in Brazil with the main participants. This will continue with on site visits anticipated in the future.