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United States Department of Agriculture

Agricultural Research Service

2009 Annual Report

1a.Objectives (from AD-416)
Assemble and analyze a public genetic database for soybeans and wheat. Through collaborative efforts, the potential value of these crops will be explored. Support breeders by improving near infrared and gas chromatographic methods for rapid analyses of soybean composition and soybean oil, increase accuracy and precision of analyses and report results rapidly to customers. Reestablish collaboration with the University of Illinois and Purdue University.

1b.Approach (from AD-416)
Using new wet chemical methods, improved near infrared calibrations will be performed and compared to calibrations by other NIR users. A wider range of soybean composition will be worked into the calibration. Soxhlet and nitrogen analyses from newly obtained equipment will be used in the calibration. Carbohydrate analyses will be performed on a large number of soybean samples and added to the current calibration.

3.Progress Report
Continual calibration of near infrared (NIR) methods and the use of routine standards has improved the quality of analysis generated for thousands of soybean samples provided by breeders. Congressionally mandated Specific Cooperative Agreements (SCA) with the University of Illinois and Purdue University for improving soybean and wheat genetics were renewed in July of 2008 and continued through 2010. These SCA projects are developing improved disease resistance in wheat and soybean. NIR calibrations were developed for the determination of phytochemicals in ground mint plant material and this calibration has been used to support work in CRIS 3620-41000-125-00D. In 2009, work was initiated to develop a non-destructive rapid single seed method for the determination of seed viability in collaboration with the North Central Regional Plant Introduction Station in Ames, IA. Initial efforts have shown limited success.

1. PROVIDED ANALYTICAL SUPPORT FOR BREEDERS THROUGHOUT THE U.S. Soybean breeders are dependent on rapid, standardized and accurate chemical properties to guide their breeding efforts. In 2009, we saw a continual increase in utilization of this analysis program with oil, protein and moisture content determinations rising to 39,675 soybean samples by NIR, as well as fatty acid profiles of 15,880 samples. This data was compiled and published in the Annual USDA publication, Coordinated Soybean Analysis. All of this data has been provided to breeders throughout the U.S. where this information is critical to the development of improved soybean varieties.

Last Modified: 5/5/2015
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