2011 Annual Report
1a.Objectives (from AD-416)
The objective of this cooperative research project is to investigate host selection by the Asian Longhorned Beetle (ALB), ANOPLOPHORA GLABRIPENNIS; develop an understanding of its chemical ecology within the context of its behavioral ecology; and develop technologies for prediction, detection, monitoring, management and control of adult ALB (e.g. artificial lure).
1b.Approach (from AD-416)
Complementary field and laboratory studies will be conducted. (1) Host tree volatiles emanating from ALB host tree species will be collected under natural field conditions (aerations and extractions) and will be characterized using GC-MS, GC-EAD and other appropriate analytical procedures at Simon Fraser University (SFU). (2) Adult ALB behavioral responsiveness to formulated blends will be evaluated in an olfactometer under laboratory conditions at BIIRU. (3) Adult ALB attraction to EAD and olfactometer active blends will be evaluated under natural field conditions in China, including evaluation of blend composition and component ratio, dose, release devise, attractive radius and attraction efficiency. This research will collectively complement of our ongoing investigations of host selection by ALB, and will result in the identification of artificial lures, and protocols for utilizing these tools for prediction, detection, monitoring, management and eradication of ALB.
We completed field studies and analysis of data in November 2010. In these field studies we evaluated the relative attraction of ALB to: (a) different dosages of host odors previously shown to be attractive in 2008 studies; (b) pure vs. mixtures of isomers of a key component of host odors; and (c) host odors vs. host odor with the addition of attractants produced by the beetle. Results showed: (1) increased ALB attraction in response to increasing release rates of host odors (volatiles) produced by Painted Maple; (2) no difference in ALB attraction to traps baited with pure vs. mixtures of isomers of host odors (volatiles) produced by Painted Maple; (3) greater attraction of ALB to traps baited with a combination of odors (volatiles) release by Painted Maple and male ALB beetles than traps baited with either one of the two odors; and (4) ca. 99% of ALB attracted to all baited traps were female beetles. We initiated preliminary studies to evaluate ALB attraction to host odors vs. host odor with the addition of attractants produced by ALB in the existing ALB infestation in Worcester, MA. Most notably, these studies are designed to simultaneously evaluate the implementation (technology transfer) of the lures for early detection of ALB in the ongoing eradication program in Worcester, MA. Preliminary implementation was also initiated in ALB infestations in Toronto, Canada, and Cornuda, Italy. Studies are in progress at the time of this report. These studies were monitored by conference calls and email communications.