Start Date: Mar 14, 2007
End Date: Mar 13, 2012
Develop sensitive PCR-based detection methods and utilize these techniques to evaluate virus distribution in seed and plant tissues of tomato as well as other alternative crops or weed hosts. Develop molecular-based markers for identification and utilize these markers for environmental tracking of the vegetable Brassica leaf spotting bacterium Pseudomonas syringae pv. maculicola (Psm). Screen tomato germplasm for resistance to PepMV, evaluate the inheritance of resistance to Zucchini yellow mosaic virus (ZYMV) in watermelon, and develop molecular markers linked to the ZYMV resistance locus in watermelon. Screen germplasm from national collections of Brassica rapa and Brassica juncea for resistance to Pseudomonas syringae pv maculicola, and evaluate the genetics of resistance. Identify non-phytopathogenic pseudomonads that inhibit Pseudomonas syringae pv. maculicola and test for efficacy as biological control agents. Identify bacterial genes involved in bacterial-biocontrol colonization of plants using full-genome microarray analysis. Develop an effective seed treatment method for PepMV in tomato seed and generate virus-free materials of heirloom sweetpotato germplasm and breeding materials. Test effectiveness of the nematode-ovicidal bacterium Pseudomonas synxantha BG33R against root-knot nematode on melon in greenhouse and field assays.