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United States Department of Agriculture

Agricultural Research Service

Research Project: GENOMIC APPROACHES TO ADDING VALUE TO AGRICULTURE

Location: Functional Foods Research Unit

Project Number: 3620-43000-007-01
Project Type: Specific Cooperative Agreement

Start Date: Sep 12, 2006
End Date: Sep 11, 2011

Objective:
To decipher the networks of genes expressed during soybean seed development using the latest methods of high throughput sequencing of messenger RNAs from genetic lines that vary in protein and oil composition. The long range goals are to discover the genes that shape important plant traits in soybean that make it valuable as a source of protein and oil and to aid in keeping soybean competitive in U.S. and world markets.

Approach:
The following experimental approaches will be used: (1) to conduct high throughput sequencing of the mRNAs from developing seed of genetic lines that vary in protein and oil content to obtain over 10 million sequence reads of at least 70 bases for each sample; (2) to quantify gene expression and align the sequences to the soybean genome; (3) to annotate the differentially expressed genes; (4) to confirm differential gene expression with an independent method; and (5) to deduce gene expression networks and physiological models for how the protein and oil levels are partitioned in the high versus low protein or oil lines.

Last Modified: 8/27/2014
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