Location: Functional Foods Research Unit
Project Number: 3620-43000-007-01
Start Date: Sep 12, 2006
End Date: Sep 11, 2011
The following experimental approaches will be used: (1) to conduct high throughput sequencing of the mRNAs from developing seed of genetic lines that vary in protein and oil content to obtain over 10 million sequence reads of at least 70 bases for each sample; (2) to quantify gene expression and align the sequences to the soybean genome; (3) to annotate the differentially expressed genes; (4) to confirm differential gene expression with an independent method; and (5) to deduce gene expression networks and physiological models for how the protein and oil levels are partitioned in the high versus low protein or oil lines.