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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR & BIOCHEMICAL DETECTION & INTERVENTION METHODS FOR BACTERIAL AND VIRAL PATHOGENS IN AQUACULTURE PRODUCTS Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)

Light scattering sensor for real-time identification of Vibrio parahaemolyticus, V. vulnificus and V. cholera colonies on solid agar plates - (Peer Reviewed Journal)
Huff, K., Aroonnual, A., Bae, E., Banada, P., Rajwa, B., Rajwa, B., Hirleman, E.D., Robinson, J.P., Richards, G.P., Bhunia, A. 2012. Light scattering sensor for real-time identification of Vibrio parahaemolyticus, V. vulnificus and V. cholera colonies on solid agar plates. Microbial Biotechnology. DOI: 10.1111/j.1751-7915.2012.00349.x.
Water quality parameters and total aerobic bacterial and vibrionaceae loads in eastern oysters (Crassostrea virginica) from oyster gardening sites - (Peer Reviewed Journal)
Fay, J., Richards, G.P., Ozbay, G. 2012. Water quality parameters and total aerobic bacterial and vibirionaceae loads in eastern oysters (crassostrea virginica) from oyster gardening sites. Archives of Environmental Contamination and Toxicology. 64:628-637.
Rapid extraction of virus-contaminated hemocytes from oysters - (Peer Reviewed Journal)
(17-Sep-11)
Hemocytes are sites of persistence for virus-contaminated oysters - (Peer Reviewed Journal)
Provost, K., Ozbay, G., Anderson, R., Richards, G.P., Kingsley, D.H. 2011. Hemocytes are sites of persistence for virus-contaminated oysters. Applied and Environmental Microbiology. 77:8360-8369.
New perspectives on virus detection in shellfish: hemocytes as a source of concentrated virus - (Abstract Only)
(13-Jun-11)
Human norovirus inactivation in oysters by high hydrostatic pressure processing: A randomized double-blinded study - (Peer Reviewed Journal)
Leon, J., Kingsley, D.H., Montes, J., Richards, G.P., Lyon, G., Abdulhafid, G., Seitz, S., Fernandez, M., Teunis, P.F., Flick, G.J., Moe, C.L. 2011. Human norovirus inactivation in oysters by high hydrostatic pressure processing: A randomized double-blinded study. Journal of Infectious Diseases. 77(15):5476-82.
USDA Food-Borne Virus Research Initiatives at Delaware State University - (Abstract Only)
(09-Apr-11)
Mechanisms of viral persistence within bivalves - (Abstract Only)
(17-Dec-10)
High pressure processing as an intervention for raw virus-contaminated shellfish - (Abstract Only)
(17-Dec-10)
New targets for expedient detection of viruses within shellfish - (Abstract Only)
Kingsley, D.H. 2011. New targets for expedient detection of viruses within shellfish [abstract]. 1st International Symposium on Fisheries, Agriculture and Food Hygiene. February 20-28, 2011, Lima,Peru. p.1.
Inaccuracies in predicting human norovirus inactivation using surrogate viruses - (Abstract Only)
Richards, G.P. 2011. Inaccuracies in predicting human norovirus inactivation using surrogate viruses. Journal of Shellfish Research, Volume 30, Issue 2, P. 548.
Development of rapid hemocyte-based extraction methods for detection of hepatitis A virus and murine norovirus in contaminated oysters - (Abstract Only)
Dancho,B.,Kingsley,D.2010.Development of rapid hemocyte-based extraction methods for detection of hepatitis A virus and murine norovirus in contaminated oysters [abstract].Interstate Seafood Seminar.Ocean City,MD.p.1.
Persistence of enteric viruses within oysters (Crassostrea virginica) - (Abstract Only)
Kingsley, D. 2010. Persistance of enteric viruses within oysters (Crassostrea virginica) [abstract].Interstate Seafood Seminar.Ocean City,MD.p.1.
High hydrostatic pressure processing of murine norovirus 1-contaminated oysters inhibits oral infection in STAT-1 -/- deficient female mice - (Peer Reviewed Journal)
Gogal, R.M., Kerr, R., Kingsley, D.H., Granata, L.A., Leroith, T., Holliman, S.D., Dancho, B.A., Flick, G.J. 2011. High hydrostatic pressure processing of murine norovirus 1-contaminated oysters inhibits oral infection in STAT-1 -/- deficient female mice. Journal of Food Protection. 74:209-214.
Potential virus detection and intervention methods for molluscan shellfish - (Abstract Only)
Kingsley, D.H. 2010. Potential virus detection and intervention methods for molluscan shellfish. [abstract]. Indiana Molecular Biology Institute, Indiana University. p. 1.
Pursuit of methods to propagate norovirus: decades of research - (Proceedings)
Richards, G.P. 2010. Pursuit of methods to propagate norovirus: decades of research. Pursuit of human norovirus propagation methods: decades of research. Edited by P.Lassus. Proceedings of the 7th International Conf. on Molluscan Shellfish Safety, Nantes, France, 14-19, June, 2009, pages 100-107
High pressure processing inactivates human norovirus within oysters - (Abstract Only)
Kingsley, D.H., Leon, J.S., Richards, G.P., Flick, G., Lyon, G.M., Abdulhafid, G., Sobolik, J., Creadore, A., Seitz, S., Smith, E., Fernandez, M., Moe, C. 2010. High pressure processing inactivates human norovirus within oysters [abstract]. Annual Meeting at American Society for Virology. p. 1.
A Potential Mechanism of Virus Persistence within Bivalve Shellfish. - (Abstract Only)
Kingsley,D.,Provost,K. 2010.A Potential Mechanism of Virus Persistance within Bivalve Shellfish [abstract].American Society of Virology.Bozeman,Montana.p.1.
Rapid detection of hepatitis A virus and murine norovirus in hemocytes of contaminated oysters - (Abstract Only)
Dancho,B.,Kingsley,D. 2010. Rapid detection of hepatitis A virus and murine norovirus in hemocytes of contaminated oysters [abstract].American Society of Virology.Bozeman, Montana. p.1.
Analysis of norovirus contamination of seafood - (Abstract Only)
Arntzen, C., Kingsley, D.H., Herbst-Kralovetz, M., Lay, M., Sarker, S., Atmar, R., Estes, M., Nickerson, C. 2010. Analysis of norovirus contamination of seafood. [abstract]. Institute of Food Technologists. Annual Meeting and NIFA NRI/AFRI Meeting. p. 1.
Retention of Enteric Viruses by the Hemocytes of the Eastern Oyster (Crassostrea virginica) - (Abstract Only)
Kingsley,D.,Provost,K.,Anderson,R.,Ozbay,G.2010.Retention of enteric viruses by the hemocytes of the Eastern Oyster (Crassostrea virginica)[abstract].IFT Annual Meeting.Chicago,IL.p.1.
Retention of Enteric Viruses by the Hemocytes of the Eastern Oyster (Crassostrea virginica) - (Abstract Only)
Ozbay,G.,Kingsley,D.,Provost,K.,Anderson,R.2010.Retention of Enteric Viruses by the Hemocytes of the Eastern Oyster (Crassostrea virginica)[abstract].Institute of Food Technologists Meeting. Chicago,IL.p.1.
Novel Platform Technologies for Analysis of Norovirus Contamination of Sea Food - (Abstract Only)
Arntzenl,C.,Kingsley,D.,Herbst-Kralovetz,M.,Sarker,S.,Dienhalt,C.,Nickerson,C. 2010. Novel platform technologies for anaylsis of Norovirus contamination of Seafood [abstract].IFT Meeting.Chicago,IL. p.1.
High Pressure Inactivation of HAV within Mussels - (Peer Reviewed Journal)
Terio, V., Tantillo, G., Martella, V., Dipinto, P., Buonavoglia, C., Kingsley, D.H. 2010. High Pressure Inactivation of HAV within Mussels. Food and Environmental Virology. 2:83-88.
Growth of Vibrio parahaemolyticus O3:K6 at Different Salt Concentrations Modulates Responses to pH and Temperature Stresses - (Peer Reviewed Journal)
Whitaker, B.W., Michelle, P.A., Naughton, L.M., Richards, G.P., Blumerman, S.L., Boyd, F.E. 2010. Growth of Vibrio parahaemolyticus O3:K6 at Different Salt Concentrations Modulates Responses to pH and Temperature Stresses. Applied and Environmental Microbiology. 76:720-729.
Processing Strategies to Inactivate Enteric Viruses in Shellfish: Limitations of Surrogate Viruses and Molecular Methods - (Peer Reviewed Journal)
Richards, G.P., Macleod, C., Le Guyader, F.S. 2010. Processing Strategies to Inactivate Enteric Viruses in Shellfish: Limitations of Surrogate Viruses and Molecular Methods. Food and Environmental Virology. Available: doi:10.107/s12560-010-9045-2.
Fluorogenic membrane overlays to enumerate total coliforms, Escherichia coli, and total Vibrionaceae in shellfish and seawater - (Peer Reviewed Journal)
Richards, G.P., Watson, M.A. 2010. Fluorogenic membrane overlays to enumerate total coliforms, Escherichia coli, and total Vibrionaceae in shellfish and seawater. Internationl Journal of Microbiology. Vol 2010, Article ID 910486, http://www.hindawi.com/journals/ijmb/2010/910486.html.
Challenges for Norovirus Detection within Shellfish - (Abstract Only)
Kingsley,D.,2009.Challenges for Novovirus detection within shellfish [abstract].Purdue/ARS Meeting.West Lafayette, Indiana.p.1.
Norovirus: Human Health and Food-borne Implications - (Abstract Only)
Kingsley,D.2009.Norovirus: Human Health and Foodborne implications [abstract].Anne Arundel County Health Laboratory.Annapolis,MD. p.1.
High pressure inactivation of HAV within oysters: comparison of shucked oysters with whole in shell meats - (Peer Reviewed Journal)
Kingsley, D.H., Calci, K., Holliman, S., Dancho, B.A., Flick, G. 2009. High pressure inactivation of HAV within oysters: comparison of shucked oysters with whole in shell meats. Food and Environmental Virology. 1:137-140.
Food-Borne Noroviruses - (Book / Chapter)
(17-Jun-09)
Detection, isolation, and persistence of viruses within bivalve mollusks - (Abstract Only)
Kingsley,D. 2009.Detection,isolation and persistance of viruses within bivalve mollusks [abstract]. University of Bari,Italy. p. 1.
Inactivation of HAV and norovirus surrogates within raw shellfish and other foods - (Abstract Only)
Kingsley,D. 2009. Inactivateion of HAV and norovirus surrogates within raw shellfish and other foods [abstract]. University of Bari,Italy. p.1.
Influence of pH, Salt and Temperature on Pressure Inactivation of Hepatitis A virus - (Peer Reviewed Journal)
Kingsley, D.H., Chen, H. 2009. Influence of pH, Salt and Temperature on Pressure Inactivation of Hepatitis A virus. Food Microbiology. 130:61-64.
The Influence of Feral Horse Activity on Water and Shellfish (Gukensia demissa) Quality Along the Western Coast of Assateague Island National Seashore, Maryland - (Peer Reviewed Journal)
Lambert, M.S., Ozbay, G., Richards, G.P. 2009. The Influence of Feral Horse Activity on Water and Shellfish (Gukensia demissa) Quality Along the Western Coast of Assateague Island National Seashore, Maryland. Estuaries and Coasts - Journal of the Estuarine Research Federation. 57:405-415.
Evaluation of High Pressure Effects on Food-borne Viruses - (Proceedings)
(10-Nov-08)
High Pressure Inactivation of Food-borne Viruses - (Abstract Only)
Kingsley,D. 2008.High Pressure Inactivation of Foodborne viruses [abstract].Conference on High Pressure Bioscience and Biotechnology.LaJolla,CA.p.1.
An Overview of Enteric Virus Extraction and Assay Methods - (Proceedings)
Richards, G.P. 2009. AN OVERVIEW OF ENTERIC VIRUS EXTRACTION AND ASSAY METHODS. In:P.Busby (ed)., Proceedings of the 6th Int'l Conference on Molluscan Shellfish Safety, Blenheim, New Zealand. pp.111-118.
Pressure Inactivation of Vibrio parahaemolyticus in Oysters- Influence of Pressure level and Treatment Temperature - (Peer Reviewed Journal)
Kural, A.G., Shearer, A., Kingsley, D.H., Chen, H. 2008. Pressure Inactivation of Vibrio parahaemolyticus in Oysters- Influence of Pressure level and Treatment Temperature. International Journal of Food Microbiology. 127:1-5.
Persistance of Viruses in Oyster (Crassostrea Virginica) Hemocytes - (Abstract Only)
Ozbay,G.,Kingsley,D.2008 Persistance of Viruses in Oyster (Crassostrea Virginica)Hemocytes.Join 1890 AEA/ARD Land Grant Conference.Memphis,TN. p.1.
Shewanella and Photobacterium in Oysters and Seawater from the Delaware Bay - (Peer Reviewed Journal)
Richards, G.P., Watson, M.A., Crane, E.J., Bushek, D., Burt, I. 2008. Shewanella and photobacterium in oysters and seawater from the delaware bay. Applied and Environmental Microbiology. 74(11):3323-3327.
Aqueous Matrix Compositions Influence Virus Inactivation by High Pressure Processing - (Peer Reviewed Journal)
Kingsley, D.H., Chen, H. 2008. Aqueous Matrix Compositions Influence Virus Inactivation by High Pressure Processing. Journal of Food Protection. 71(1):598-603.
An Rna Extraction Protocol for Shellfish-Borne Viruses - (Peer Reviewed Journal)
Kingsley, D.H. 2007. An rna extraction protocol for shellfish-borne viruses. Journal of Virological Methods. Vol.141:58-62.
Inactivation of a Norovirus by High Pressure Processing - (Peer Reviewed Journal)
Kingsley, D.H., Holliman, D., Calci, K., Chen, H., Flich, G. 2007. Inactivation of a Norovirus by High Pressure Processing. Applied and Environmental Microbiology. 73:581-585.
An Overview of Virus Extraction and Assay Methods - (Abstract Only)
Richards, G.P. 2007. An overview of virus extraction and assay methods. Abstract. 6th International Conference on Molluscan Shellfish Safety, Blenheim, New Zealand. p.78.
Last Modified: 12/21/2014
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