Start Date: May 19, 2006
End Date: Jan 31, 2011
Identification of gross and histological organ changes in tissues collected during feed withdrawal and alternative molting methods and effect of molting methods on S. enteritidis binding and penetration of tissues. Molecular examination of alterations in cytokine, neuroendocrine, and endocrine hormone expression and expression of these receptors in immune cells collected from hens molted with feed withdrawal and alternative molt techniques. Measurement of serum endocrine hormone alterations in hens molted with feed withdrawal and alternative molting techniques. Examination of the effect of endocrine hormone treatment and molting methods on immune cell activity, as measured by cytokine and hormone molecular expression and expression of cytokine and hormone receptor genes. Measurement of immune responses in S. enteritidis infected hens molted with feed withdrawal and alternative molting techniques.